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Pesticides, extensively used in agriculture production, have received enormous attention because of their potential threats to the environment and human health. Hence, in this study, a kind of highly sensitive and stable hybrid surface-enhanced Raman scattering (SERS)-active substrates constructed with flower-like two-dimensional molybdenum sulfide and Ag (MoS₂@Ag) has been developed, and then the above substrate was sequentially utilized in the recyclable detection of pesticide residues on several kinds of fruits and vegetables. In the first place, the excellent photocatalytic performance of the MoS₂@Ag hybrid substrate was demonstrated, which was attributed to the inhibition of electron–hole combination after the formation of Schottky barrier between the Ag NPs and MoS₂ matrix. Thereafter, two calibration curves with ultra-low limits of detection (LOD) as 6.4 × 10^–7 and 9.8 × 10^–7 mg/mL were …