Homology-Integrated CRISPR–Cas (HI-CRISPR) System for One-Step Multigene Disruption in Saccharomyces cerevisiae Z Bao, H Xiao, J Liang, L Zhang, X Xiong, N Sun, T Si, H Zhao ACS synthetic biology 4 (5), 585-594, 2015 | 423 | 2015 |
Customized optimization of metabolic pathways by combinatorial transcriptional engineering J Du, Y Yuan, T Si, J Lian, H Zhao Nucleic acids research 40 (18), e142-e142, 2012 | 262 | 2012 |
Design and construction of acetyl-CoA overproducing Saccharomyces cerevisiae strains J Lian, T Si, NU Nair, H Zhao Metabolic engineering 24, 139-149, 2014 | 251 | 2014 |
Automated multiplex genome-scale engineering in yeast T Si, R Chao, Y Min, Y Wu, W Ren, H Zhao Nature communications 8 (1), 15187, 2017 | 217 | 2017 |
Biocatalyst development by directed evolution M Wang, T Si, H Zhao Bioresource technology 115, 117-125, 2012 | 189 | 2012 |
Engineering biological systems using automated biofoundries R Chao, S Mishra, T Si, H Zhao Metabolic Engineering 42, 98-108, 2017 | 178 | 2017 |
Development of a Synthetic Malonyl-CoA Sensor in Saccharomyces cerevisiae for Intracellular Metabolite Monitoring and Genetic Screening S Li, T Si, M Wang, H Zhao ACS synthetic biology 4 (12), 1308-1315, 2015 | 178 | 2015 |
Directed evolution: an evolving and enabling synthetic biology tool RE Cobb, T Si, H Zhao Current opinion in chemical biology 16 (3-4), 285-291, 2012 | 139 | 2012 |
Metabolic engineering of a synergistic pathway for n-butanol production in Saccharomyces cerevisiae S Shi, T Si, Z Liu, H Zhang, EL Ang, H Zhao Scientific reports 6 (1), 25675, 2016 | 119 | 2016 |
Utilizing an endogenous pathway for 1-butanol production in Saccharomyces cerevisiae T Si, Y Luo, H Xiao, H Zhao Metabolic engineering 22, 60-68, 2014 | 110 | 2014 |
Direct conversion of xylan to ethanol by recombinant Saccharomyces cerevisiae strains displaying an engineered minihemicellulosome J Sun, F Wen, T Si, JH Xu, H Zhao Applied and Environmental Microbiology 78 (11), 3837-3845, 2012 | 102 | 2012 |
RNAi-Assisted Genome Evolution in Saccharomyces cerevisiae for Complex Phenotype Engineering T Si, Y Luo, Z Bao, H Zhao ACS synthetic biology 4 (3), 283-291, 2015 | 85 | 2015 |
Profiling of microbial colonies for high-throughput engineering of multistep enzymatic reactions via optically guided matrix-assisted laser desorption/ionization mass spectrometry T Si, B Li, TJ Comi, Y Wu, P Hu, Y Wu, Y Min, DA Mitchell, H Zhao, ... Journal of the American Chemical Society 139 (36), 12466-12473, 2017 | 69 | 2017 |
Engineered pentafunctional minicellulosome for simultaneous saccharification and ethanol fermentation in Saccharomyces cerevisiae Y Liang, T Si, EL Ang, H Zhao Applied and environmental microbiology 80 (21), 6677-6684, 2014 | 67 | 2014 |
Fully automated one-step synthesis of single-transcript TALEN pairs using a biological foundry R Chao, J Liang, I Tasan, T Si, L Ju, H Zhao ACS synthetic biology 6 (4), 678-685, 2017 | 65 | 2017 |
Towards achieving a flattop crystal size distribution by continuous seeding and controlled growth M Jiang, MH Wong, Z Zhu, J Zhang, L Zhou, K Wang, ANF Versypt, T Si, ... Chemical engineering science 77, 2-9, 2012 | 61 | 2012 |
Accelerating strain engineering in biofuel research via build and test automation of synthetic biology J Zhang, Y Chen, L Fu, E Guo, B Wang, L Dai, T Si Current Opinion in Biotechnology 67, 88-98, 2021 | 57 | 2021 |
A brief overview of synthetic biology research programs and roadmap studies in the United States T Si, H Zhao Synthetic and systems biotechnology 1 (4), 258-264, 2016 | 55 | 2016 |
A one‐step matrix application method for MALDI mass spectrometry imaging of bacterial colony biofilms B Li, TJ Comi, T Si, SJB Dunham, JV Sweedler Journal of mass spectrometry 51 (11), 1030-1035, 2016 | 55 | 2016 |
Characterization of Bacillus subtilis Colony Biofilms via Mass Spectrometry and Fluorescence Imaging T Si, B Li, K Zhang, Y Xu, H Zhao, JV Sweedler Journal of Proteome Research 15 (6), 1955-1962, 2016 | 53 | 2016 |