Crossing enhanced and high fidelity SpCas9 nucleases to optimize specificity and cleavage PI Kulcsár, A Tálas, K Huszár, Z Ligeti, E Tóth, N Weinhardt, E Fodor, ... Genome biology 18, 1-17, 2017 | 138 | 2017 |
Improved LbCas12a variants with altered PAM specificities further broaden the genome targeting range of Cas12a nucleases E Toth, E Varga, PI Kulcsár, V Kocsis-Jutka, SL Krausz, A Nyeste, ... Nucleic acids research 48 (7), 3722-3733, 2020 | 103 | 2020 |
Mb-and FnCpf1 nucleases are active in mammalian cells: activities and PAM preferences of four wild-type Cpf1 nucleases and of their altered PAM specificity variants E Tóth, BC Czene, PI Kulcsár, SL Krausz, A Tálas, A Nyeste, É Varga, ... Nucleic acids research 46 (19), 10272-10285, 2018 | 67 | 2018 |
Blackjack mutations improve the on-target activities of increased fidelity variants of SpCas9 with 5′ G-extended sgRNAs PI Kulcsár, A Tálas, E Tóth, A Nyeste, Z Ligeti, Z Welker, E Welker Nature communications 11 (1), 1223, 2020 | 40 | 2020 |
A convenient method to pre-screen candidate guide RNAs for CRISPR/Cas9 gene editing by NHEJ-mediated integration of a ‘self-cleaving’GFP-expression plasmid A Tálas, PI Kulcsár, N Weinhardt, A Borsy, E Tóth, K Szebenyi, SL Krausz, ... DNA Research 24 (6), 609-621, 2017 | 28 | 2017 |
SuperFi-Cas9 exhibits remarkable fidelity but severely reduced activity yet works effectively with ABE8e PI Kulcsár, A Tálas, Z Ligeti, SL Krausz, E Welker Nature Communications 13 (1), 6858, 2022 | 22 | 2022 |
Position-dependent sequence motif preferences of SpCas9 are largely determined by scaffold-complementary spacer motifs K Huszár, Z Welker, Z Györgypál, E Tóth, Z Ligeti, PI Kulcsár, J Dancsó, ... Nucleic Acids Research 51 (11), 5847-5863, 2023 | 2 | 2023 |
Application of a Receptor-Binding-Domain-Based Simple Immunoassay for Assessing Humoral Immunity against Emerging SARS-CoV-2 Virus Variants O Mózner, J Moldvay, KS Szabó, D Vaskó, J Domján, D Ács, Z Ligeti, ... Biomedicines 11 (12), 3193, 2023 | 1 | 2023 |
A cleavage rule for selection of increased-fidelity SpCas9 variants with high efficiency and no detectable off-targets PI Kulcsár, A Tálas, Z Ligeti, E Tóth, Z Rakvács, Z Bartos, SL Krausz, ... Nature Communications 14 (1), 5746, 2023 | 1 | 2023 |
Application of an Rbd-Based Simple Immunoassay for Assessing Humoral Immunity against Emerging SARS-CoV-2 Virus Variants O Mózner, J Moldvay, KS Szabó, D Vaskó, J Domján, D Ács, Z Ligeti, ... Preprints, 2023 | | 2023 |