Emergent Properties of Reduced-Genome Escherichia coli G Posfai, G Plunkett III, T Fehér, D Frisch, GM Keil, K Umenhoffer, ... science 312 (5776), 1044-1046, 2006 | 855 | 2006 |
Effects of elevated ammonium on glycosylation gene expression in CHO cells P Chen, SW Harcum Metabolic Engineering 8 (2), 123-132, 2006 | 158 | 2006 |
Effects of amino acid additions on ammonium stressed CHO cells P Chen, SW Harcum Journal of Biotechnology 117 (3), 277-286, 2005 | 148 | 2005 |
Transcriptome profiles for high‐cell‐density recombinant and wild‐type Escherichia coli FT Haddadin, SW Harcum Biotechnology and bioengineering 90 (2), 127-153, 2005 | 100 | 2005 |
Xanthan gum production from waste sugar beet pulp SD Yoo, SW Harcum Bioresource Technology 70 (1), 105-109, 1999 | 99 | 1999 |
Response dynamics of 26‐, 34‐, 39‐, 54‐, and 80‐kDa proteases in induced cultures of recombinant Escherichia coli SW Harcum, WE Bentley Biotechnology and bioengineering 42 (6), 675-685, 1993 | 97 | 1993 |
Recombinant protein production in an Escherichia coli reduced genome strain SS Sharma, FR Blattner, SW Harcum Metabolic engineering 9 (2), 133-141, 2007 | 93 | 2007 |
Intein-mediated protein purification of fusion proteins expressed under high-cell density conditions in E. coli SS Sharma, S Chong, SW Harcum Journal of biotechnology 125 (1), 48-56, 2006 | 75 | 2006 |
Heat-shock and stringent responses have overlapping protease activity in Escherichia coli Implications for heterologous protein yield SW Harcum, WE Bentley Applied biochemistry and biotechnology 80, 23-37, 1999 | 72 | 1999 |
Genomics in mammalian cell culture bioprocessing DM Wuest, SW Harcum, KH Lee Biotechnology advances 30 (3), 629-638, 2012 | 71 | 2012 |
Temperature effects on product‐quality‐related enzymes in batch CHO cell cultures producing recombinant tPA KJR Clark, FWR Chaplin, SW Harcum Biotechnology progress 20 (6), 1888-1892, 2004 | 61 | 2004 |
Global transcriptome response of recombinant Escherichia coli to heat-shock and dual heat-shock recombinant protein induction SW Harcum, FT Haddadin Journal of Industrial Microbiology and Biotechnology 33 (10), 801-814, 2006 | 58 | 2006 |
Gene‐expression profiles for five key glycosylation genes for galactose‐fed CHO cells expressing recombinant IL‐4/13 cytokine trap KJR Clark, J Griffiths, KM Bailey, SW Harcum Biotechnology and bioengineering 90 (5), 568-577, 2005 | 49 | 2005 |
Dynamic transcriptional response of Escherichia coli to inclusion body formation F Baig, LP Fernando, MA Salazar, RR Powell, TF Bruce, SW Harcum Biotechnology and bioengineering 111 (5), 980-999, 2014 | 41 | 2014 |
Optimal nutrient feed policies for heterologous protein production SW Harcum, DM Ramirez, WE Bentley Applied biochemistry and biotechnology 34, 161-173, 1992 | 38 | 1992 |
Expression of two recombinant chloramphenicol acetyltransferase variants in highly reduced genome Escherichia coli strains SS Sharma, JW Campbell, D Frisch, FR Blattner, SW Harcum Biotechnology and bioengineering 98 (5), 1056-1070, 2007 | 36 | 2007 |
Detection, quantification, and characterization of proteases in recombinant Escherichia coli SW Harcum, WE Bentley Biotechnology techniques 7 (6), 441-447, 1993 | 32 | 1993 |
High extracellular lactate causes reductive carboxylation in breast tissue cell lines grown under normoxic conditions AN Brodsky, DC Odenwelder, SW Harcum PloS one 14 (6), e0213419, 2019 | 29 | 2019 |
Protein glycosylation SW Harcum Cell culture technology for pharmaceutical and cell-based therapies, 131-172, 2005 | 24 | 2005 |
Structured model to predict intracellular amino acid shortages during recombinant protein overexpression in E. coli SW Harcum Journal of biotechnology 93 (3), 189-202, 2002 | 23 | 2002 |