Consequences of Cas9 cleavage in the chromosome of Escherichia coli L Cui, D Bikard Nucleic acids research 44 (9), 4243-4251, 2016 | 286 | 2016 |
One-step cloning and chromosomal integration of DNA F St-Pierre, L Cui, DG Priest, D Endy, IB Dodd, KE Shearwin ACS synthetic biology 2 (9), 537-541, 2013 | 247 | 2013 |
A CRISPRi screen in E. coli reveals sequence-specific toxicity of dCas9 L Cui, A Vigouroux, F Rousset, H Varet, V Khanna, D Bikard Nature communications 9 (1), 1912, 2018 | 243 | 2018 |
Genome-wide CRISPR-dCas9 screens in E. coli identify essential genes and phage host factors F Rousset, L Cui, E Siouve, C Becavin, F Depardieu, D Bikard PLoS genetics 14 (11), e1007749, 2018 | 199 | 2018 |
Tuning dCas9's ability to block transcription enables robust, noiseless knockdown of bacterial genes ST Antoine Vigouroux, Enno Oldewurtel, Lun Cui, David Bikard Molecular Systems Biology, 2018 | 109 | 2018 |
Improving sequence-specific antimicrobials by blocking dna repair D Bikard, CUI Lun, X Duportet, JF Rodriguez | 95 | 2017 |
Inhibition of NHEJ repair by type II-A CRISPR-Cas systems in bacteria A Bernheim, A Calvo-Villamañán, C Basier, L Cui, EPC Rocha, ... Nature communications 8 (1), 2094, 2017 | 90 | 2017 |
Quantitation of the DNA tethering effect in long-range DNA looping in vivo and in vitro using the Lac and λ repressors DG Priest, L Cui, S Kumar, DD Dunlap, IB Dodd, KE Shearwin Proceedings of the National Academy of Sciences 111 (1), 349-354, 2014 | 54 | 2014 |
On-target activity predictions enable improved CRISPR–dCas9 screens in bacteria A Calvo-Villamañán, JW Ng, R Planel, H Ménager, A Chen, L Cui, ... Nucleic acids research 48 (11), e64-e64, 2020 | 49 | 2020 |
Enhancer-like long-range transcriptional activation by λ CI-mediated DNA looping L Cui, I Murchland, KE Shearwin, IB Dodd Proceedings of the National Academy of Sciences 110 (8), 2922-2927, 2013 | 44 | 2013 |
Cas9 off-target binding to the promoter of bacterial genes leads to silencing and toxicity W Rostain, T Grebert, D Vyhovskyi, PT Pizarro, ... Nucleic Acids Research 51 (7), 3485-3496, 2023 | 29 | 2023 |
Within-patient evolution of plasmid-mediated antimicrobial resistance J DelaFuente, L Toribio-Celestino, A Santos-Lopez, R León-Sampedro, ... Nature ecology & evolution 6 (12), 1980-1991, 2022 | 27 | 2022 |
A CRISPRi screen in E. coli reveals sequence-specific toxicity of dCas9. Nat Commun 9: 1912 L Cui, A Vigouroux, F Rousset, H Varet, V Khanna, D Bikard | 21 | 2018 |
Lipoprotein DolP supports proper folding of BamA in the bacterial outer membrane promoting fitness upon envelope stress D Ranava, Y Yang, L Orenday-Tapia, F Rousset, C Turlan, V Morales, ... Elife 10, e67817, 2021 | 15 | 2021 |
Clonetegration using OSIP plasmids: One-step DNA assembly and site-specific genomic integration in bacteria L Cui, KE Shearwin Synthetic DNA: Methods and Protocols, 139-155, 2017 | 11 | 2017 |
Bacteriophage lambda repressor mediates the formation of a complex enhancer-like structure L Cui, I Murchland, IB Dodd, KE Shearwin Transcription 4 (5), 201-205, 2013 | 4 | 2013 |
Genome-wide CRISPR-Cas9 screen in E. coli identifies design rules for efficient targeting B Gutierrez, JW Ng, L Cui, C Becavin, D Bikard bioRxiv, 308148, 2018 | 3 | 2018 |
Repurposing site-specific recombinases for synthetic biology L Cui, F St-Pierre, K Shearwin Future Microbiology 8 (11), 1361-1364, 2013 | 2 | 2013 |
Recombinant T7 RNA polymerase production using ClearColi BL21(DE3) and animal-free media for in vitro transcription Q Liang, B Tu, L Cui Applied Microbiology and Biotechnology 108 (1), 1-15, 2024 | 1 | 2024 |
Outer membrane lipoprotein DolP interacts with the BAM complex and promotes fitness during envelope stress response D Ranava, Y Yang, L Orenday-Tapia, F Rousset, C Turlan, L Cui, ... bioRxiv, 2020.09. 23.308296, 2020 | 1 | 2020 |