Design of the linkers which effectively separate domains of a bifunctional fusion protein R Arai, H Ueda, A Kitayama, N Kamiya, T Nagamune Protein engineering 14 (8), 529-532, 2001 | 821 | 2001 |
Sortase‐mediated ligation: a gift from gram‐positive bacteria to protein engineering S Tsukiji, T Nagamune ChemBioChem 10 (5), 787-798, 2009 | 285 | 2009 |
Icariin induces osteogenic differentiation in vitro in a BMP-and Runx2-dependent manner J Zhao, S Ohba, M Shinkai, U Chung, T Nagamune Biochemical and biophysical research communications 369 (2), 444-448, 2008 | 240 | 2008 |
Open sandwich ELISA: a novel immunoassay based on the interchain interaction of antibody variable region H Ueda, K Tsumoto, K Kubota, E Suzuki, T Nagamune, H Nishimura, ... Nature Biotechnology 14 (13), 1714-1718, 1996 | 198 | 1996 |
Post-translational modification is essential for catalytic activity of nitrile hydratase T Murakami, M Nojiri, H Nakayama, M Odaka, M Yohda, N Dohmae, ... Protein Science 9 (5), 1024-1030, 2000 | 195 | 2000 |
Site‐specific protein modification on living cells catalyzed by sortase T Tanaka, T Yamamoto, S Tsukiji, T Nagamune ChemBioChem 9 (5), 802-807, 2008 | 191 | 2008 |
Protein refolding using chemical refolding additives S Yamaguchi, E Yamamoto, T Mannen, T Nagamune, T Nagamune Biotechnology journal 8 (1), 17-31, 2013 | 168 | 2013 |
Engineering of functional chimeric protein G–VargulaLuciferase Y Maeda, H Ueda, J Kazami, G Kawano, E Suzuki, T Nagamune Analytical biochemistry 249 (2), 147-152, 1997 | 152 | 1997 |
High-efficiency DNA injection into a single human mesenchymal stem cell using a nanoneedle and atomic force microscopy SW Han, C Nakamura, N Kotobuki, I Obataya, H Ohgushi, T Nagamune, ... Nanomedicine: Nanotechnology, Biology and Medicine 4 (3), 215-225, 2008 | 150 | 2008 |
Conformations of variably linked chimeric proteins evaluated by synchrotron X‐ray small‐angle scattering R Arai, W Wriggers, Y Nishikawa, T Nagamune, T Fujisawa PROTEINS: Structure, Function, and Bioinformatics 57 (4), 829-838, 2004 | 145 | 2004 |
Rapid protein anchoring into the membranes of mammalian cells using oleyl chain and poly (ethylene glycol) derivatives K Kato, C Itoh, T Yasukouchi, T Nagamune Biotechnology progress 20 (3), 897-904, 2004 | 144 | 2004 |
Activity regulation of photoreactive nitrile hydratase by nitric oxide M Odaka, K Fujii, M Hoshino, T Noguchi, M Tsujimura, S Nagashima, ... Journal of the American Chemical Society 119 (16), 3785-3791, 1997 | 135 | 1997 |
Icariin: a potential osteoinductive compound for bone tissue engineering J Zhao, S Ohba, Y Komiyama, M Shinkai, U Chung, T Nagamune Tissue Engineering Part A 16 (1), 233-243, 2010 | 130 | 2010 |
High speed polymerase chain reaction in constant flow H Nakano, K Matsuda, M Yohda, T Nagamune, I Endo, T Yamane Bioscience, biotechnology, and biochemistry 58 (2), 349-352, 1994 | 128 | 1994 |
Biomolecular engineering for nanobio/bionanotechnology T Nagamune Nano convergence 4 (1), 9, 2017 | 125 | 2017 |
Design of Ca2+‐independent Staphylococcus aureus sortase A mutants H Hirakawa, S Ishikawa, T Nagamune Biotechnology and bioengineering 109 (12), 2955-2961, 2012 | 114 | 2012 |
Photosensitive nitrile hydratase intrinsically possesses nitric oxide bound to the non-heme iron center: evidence by Fourier transform infrared spectroscopy T Noguchi, J Honda, T Nagamune, H Sasabe, Y Inoue, I Endo FEBS letters 358 (1), 9-12, 1995 | 114 | 1995 |
N-terminal glycine-specific protein conjugation catalyzed by microbial transglutaminase T Tanaka, N Kamiya, T Nagamune FEBS letters 579 (10), 2092-2096, 2005 | 112 | 2005 |
Immobilized culture of nonadherent cells on an oleyl poly (ethylene glycol) ether-modified surface K Kato, K Umezawa, DP Funeriu, M Miyake, J Miyake, T Nagamune Biotechniques 35 (5), 1014-1021, 2003 | 104 | 2003 |
Demonstration of a homogeneous noncompetitive immunoassay based on bioluminescence resonance energy transfer R Arai, H Nakagawa, K Tsumoto, W Mahoney, I Kumagai, H Ueda, ... Analytical Biochemistry 289 (1), 77-81, 2001 | 104 | 2001 |