| Crossing enhanced and high fidelity SpCas9 nucleases to optimize specificity and cleavage PI Kulcsár, A Tálas, K Huszár, Z Ligeti, E Tóth, N Weinhardt, E Fodor, ... Genome biology 18, 1-17, 2017 | 137 | 2017 |
| Improved LbCas12a variants with altered PAM specificities further broaden the genome targeting range of Cas12a nucleases E Toth, E Varga, PI Kulcsár, V Kocsis-Jutka, SL Krausz, A Nyeste, ... Nucleic acids research 48 (7), 3722-3733, 2020 | 99 | 2020 |
| Mb-and FnCpf1 nucleases are active in mammalian cells: activities and PAM preferences of four wild-type Cpf1 nucleases and of their altered PAM specificity variants E Tóth, BC Czene, PI Kulcsár, SL Krausz, A Tálas, A Nyeste, É Varga, ... Nucleic acids research 46 (19), 10272-10285, 2018 | 65 | 2018 |
| Cpf1 nucleases demonstrate robust activity to induce DNA modification by exploiting homology directed repair pathways in mammalian cells E Tóth, N Weinhardt, P Bencsura, K Huszár, PI Kulcsár, A Tálas, E Fodor, ... Biology direct 11, 1-14, 2016 | 65 | 2016 |
| Blackjack mutations improve the on-target activities of increased fidelity variants of SpCas9 with 5′ G-extended sgRNAs PI Kulcsár, A Tálas, E Tóth, A Nyeste, Z Ligeti, Z Welker, E Welker Nature communications 11 (1), 1223, 2020 | 40 | 2020 |
| A convenient method to pre-screen candidate guide RNAs for CRISPR/Cas9 gene editing by NHEJ-mediated integration of a ‘self-cleaving’GFP-expression plasmid A Tálas, PI Kulcsár, N Weinhardt, A Borsy, E Tóth, K Szebenyi, SL Krausz, ... DNA Research 24 (6), 609-621, 2017 | 27 | 2017 |
| Restriction enzyme body doubles and PCR cloning: on the general use of type IIs restriction enzymes for cloning E Tóth, K Huszár, P Bencsura, PI Kulcsár, B Vodicska, A Nyeste, Z Welker, ... PloS one 9 (3), e90896, 2014 | 20 | 2014 |
| The highly conserved, N-terminal (RXXX) 8 motif of mouse Shadoo mediates nuclear accumulation E Tóth, PI Kulcsár, E Fodor, F Ayaydin, L Kalmár, AÉ Borsy, L László, ... Biochimica et Biophysica Acta (BBA)-Molecular Cell Research 1833 (5), 1199-1211, 2013 | 13 | 2013 |
| A method for characterizing Cas9 variants via a one-million target sequence library of self-targeting sgRNAs A Tálas, K Huszár, PI Kulcsár, JK Varga, É Varga, E Tóth, Z Welker, ... Nucleic Acids Research 49 (6), e31-e31, 2021 | 12 | 2021 |
| Comparison of Anti-Shadoo Antibodies–Where is the endogenous Shadoo protein? E Tóth, E Welker International Journal of Biotechnology and Bioengineering 6 (11), 975-977, 2012 | 3 | 2012 |
| Position-dependent sequence motif preferences of SpCas9 are largely determined by scaffold-complementary spacer motifs K Huszár, Z Welker, Z Györgypál, E Tóth, Z Ligeti, PI Kulcsár, J Dancsó, ... Nucleic Acids Research 51 (11), 5847-5863, 2023 | 2 | 2023 |
| A cleavage rule for selection of increased-fidelity SpCas9 variants with high efficiency and no detectable off-targets PI Kulcsár, A Tálas, Z Ligeti, E Tóth, Z Rakvács, Z Bartos, SL Krausz, ... Nature Communications 14 (1), 5746, 2023 | 1 | 2023 |
Ervin Welkersenior scientist Biological Research Center of the Hungarian Academy of Sciences在 brc.mta.hu 的电子邮件经过验证
