| PCR amplification from single DNA molecules on magnetic beads in emulsion: application for high-throughput screening of transcription factor targets T Kojima, Y Takei, M Ohtsuka, Y Kawarasaki, T Yamane, H Nakano Nucleic acids research 33 (17), e150-e150, 2005 | 226 | 2005 |
| Crystal structure of glucansucrase from the dental caries pathogen Streptococcus mutans K Ito, S Ito, T Shimamura, S Weyand, Y Kawarasaki, T Misaka, K Abe, ... Journal of molecular biology 408 (2), 177-186, 2011 | 178 | 2011 |
| Export pathway selectivity of Escherichia coli twin arginine translocation signal peptides D Tullman-Ercek, MP DeLisa, Y Kawarasaki, P Iranpour, B Ribnicky, ... Journal of Biological Chemistry 282 (11), 8309-8316, 2007 | 173 | 2007 |
| Analyzing a dipeptide library to identify human dipeptidyl peptidase IV inhibitor VTT Lan, K Ito, M Ohno, T Motoyama, S Ito, Y Kawarasaki Food Chemistry 175, 66-73, 2015 | 131 | 2015 |
| Enhanced crossover SCRATCHY: construction and high‐throughput screening of a combinatorial library containing multiple non‐homologous crossovers Y Kawarasaki, KE Griswold, JD Stevenson, T Selzer, SJ Benkovic, ... Nucleic acids research 31 (21), e126-e126, 2003 | 90 | 2003 |
| Analysing the substrate multispecificity of a proton-coupled oligopeptide transporter using a dipeptide library K Ito, A Hikida, S Kawai, VTT Lan, T Motoyama, S Kitagawa, Y Yoshikawa, ... Nature communications 4 (1), 2502, 2013 | 87 | 2013 |
| High-throughput, cloning-independent protein library construction by combining single-molecule DNA amplification with in vitro expression S Rungpragayphan, Y Kawarasaki, T Imaeda, K Kohda, H Nakano, ... Journal of molecular biology 318 (2), 395-405, 2002 | 79 | 2002 |
| Evolution of highly active enzymes by homology-independent recombination KE Griswold, Y Kawarasaki, N Ghoneim, SJ Benkovic, BL Iverson, ... Proceedings of the National Academy of Sciences 102 (29), 10082-10087, 2005 | 69 | 2005 |
| A long-lived batch reaction system of cell-free protein synthesis Y Kawarasaki, T Kawai, H Nakano, T Yamane Analytical biochemistry 226 (2), 320-324, 1995 | 65 | 1995 |
| A periplasmic fluorescent reporter protein and its application in high-throughput membrane protein topology analysis KJ Jeong, Y Kawarasaki, J Gam, BR Harvey, BL Iverson, G Georgiou Journal of molecular biology 341 (4), 901-909, 2004 | 62 | 2004 |
| An increased rate of cell-free protein synthesis by condensing wheat-germ extract with ultrafiltration membranes H Nakano, T Tanaka, Y Kawarasaki, T Yamane Bioscience, biotechnology, and biochemistry 58 (4), 631-634, 1994 | 53 | 1994 |
| Signal peptide optimization tool for the secretion of recombinant protein from Saccharomyces cerevisiae A Mori, S Hara, T Sugahara, T Kojima, Y Iwasaki, Y Kawarasaki, T Sahara, ... Journal of bioscience and bioengineering 120 (5), 518-525, 2015 | 51 | 2015 |
| Prolonged cell-free protein synthesis in a batch system using wheat germ extract Y Kawarasaki, H Nakano, T Yamane Bioscience, biotechnology, and biochemistry 58 (10), 1911-1913, 1994 | 41 | 1994 |
| Trp-Arg-Xaa tripeptides act as uncompetitive-type inhibitors of human dipeptidyl peptidase IV VTT Lan, K Ito, S Ito, Y Kawarasaki Peptides 54, 166-170, 2014 | 40 | 2014 |
| Highly productive cell-free protein synthesis system using condensed wheat-germ extract H Nakano, T Tanaka, Y Kawarasaki, T Yamane Journal of biotechnology 46 (3), 275-282, 1996 | 39 | 1996 |
| Regulation of RraA, a protein inhibitor of RNase E-mediated RNA decay M Zhao, L Zhou, Y Kawarasaki, G Georgiou Journal of bacteriology 188 (9), 3257-3263, 2006 | 30 | 2006 |
| Chimeric gene library construction by a simple and highly versatile method using recombination‐dependent exponential amplification A Ikeuchi, Y Kawarasaki, T Shinbata, T Yamane Biotechnology progress 19 (5), 1460-1467, 2003 | 30 | 2003 |
| Purification and some properties of wheat germ acid phosphatases Y Kawarasaki, H Nakano, T Yamane Plant Science 119 (1-2), 67-77, 1996 | 30 | 1996 |
| Systematic analysis of a dipeptide library for inhibitor development using human dipeptidyl peptidase IV produced by a Saccharomyces cerevisiae expression system A Hikida, K Ito, T Motoyama, R Kato, Y Kawarasaki Biochemical and biophysical research communications 430 (4), 1217-1222, 2013 | 28 | 2013 |
| Cloning and in vitro and in vivo expression of plant glutathione S-transferase zeta class genes D Chen, Y Kawarasaki, H Nakano, T Yamane Journal of bioscience and bioengineering 95 (6), 594-600, 2003 | 28 | 2003 |
