Improvement of enzyme activity, stability and selectivity via immobilization techniques C Mateo, JM Palomo, G Fernandez-Lorente, JM Guisan, ... Enzyme and microbial technology 40 (6), 1451-1463, 2007 | 3848 | 2007 |
Modifying enzyme activity and selectivity by immobilization RC Rodrigues, C Ortiz, Á Berenguer-Murcia, R Torres, ... Chemical society reviews 42 (15), 6290-6307, 2013 | 1817 | 2013 |
Potential of different enzyme immobilization strategies to improve enzyme performance C Garcia‐Galan, Á Berenguer‐Murcia, R Fernandez‐Lafuente, ... Advanced Synthesis & Catalysis 353 (16), 2885-2904, 2011 | 1721 | 2011 |
Glutaraldehyde in bio-catalysts design: A useful crosslinker and a versatile tool in enzyme immobilization O Barbosa, C Ortiz, Á Berenguer-Murcia, R Torres, RC Rodrigues, ... Rsc Advances 4 (4), 1583-1600, 2014 | 844 | 2014 |
Control of protein immobilization: Coupling immobilization and site-directed mutagenesis to improve biocatalyst or biosensor performance K Hernandez, R Fernandez-Lafuente Enzyme and microbial technology 48 (2), 107-122, 2011 | 695 | 2011 |
Stabilization of multimeric enzymes: Strategies to prevent subunit dissociation R Fernandez-Lafuente Enzyme and microbial technology 45 (6-7), 405-418, 2009 | 682 | 2009 |
Strategies for the one-step immobilization–purification of enzymes as industrial biocatalysts O Barbosa, C Ortiz, Á Berenguer-Murcia, R Torres, RC Rodrigues, ... Biotechnology advances 33 (5), 435-456, 2015 | 665 | 2015 |
Lipase from Thermomyces lanuginosus: uses and prospects as an industrial biocatalyst R Fernandez-Lafuente Journal of Molecular Catalysis B: Enzymatic 62 (3-4), 197-212, 2010 | 656 | 2010 |
A single step purification, immobilization, and hyperactivation of lipases via interfacial adsorption on strongly hydrophobic supports A Bastida, P Sabuquillo, P Armisen, R Fernández‐Lafuente, J Huguet, ... Biotechnology and bioengineering 58 (5), 486-493, 1998 | 645 | 1998 |
Immobilization of lipases by selective adsorption on hydrophobic supports R Fernandez-Lafuente, P Armisén, P Sabuquillo, G Fernández-Lorente, ... Chemistry and physics of lipids 93 (1-2), 185-197, 1998 | 605 | 1998 |
Importance of the support properties for immobilization or purification of enzymes JCS Santos, O Barbosa, C Ortiz, A Berenguer‐Murcia, RC Rodrigues, ... ChemCatChem 7 (16), 2413-2432, 2015 | 553 | 2015 |
Novozym 435: the “perfect” lipase immobilized biocatalyst? C Ortiz, ML Ferreira, O Barbosa, JCS dos Santos, RC Rodrigues, ... Catalysis Science & Technology 9 (10), 2380-2420, 2019 | 526* | 2019 |
Heterofunctional supports in enzyme immobilization: from traditional immobilization protocols to opportunities in tuning enzyme properties O Barbosa, R Torres, C Ortiz, Á Berenguer-Murcia, RC Rodrigues, ... Biomacromolecules 14 (8), 2433-2462, 2013 | 519 | 2013 |
Immobilization of lipases on hydrophobic supports involves the open form of the enzyme EA Manoel, JCS Dos Santos, DMG Freire, N Rueda, ... Enzyme and Microbial Technology 71, 53-57, 2015 | 503 | 2015 |
Different mechanisms of protein immobilization on glutaraldehyde activated supports: effect of support activation and immobilization conditions L Betancor, F López-Gallego, A Hidalgo, N Alonso-Morales, GDOC Mateo, ... Enzyme and microbial technology 39 (4), 877-882, 2006 | 493 | 2006 |
Interfacial adsorption of lipases on very hydrophobic support (octadecyl–Sepabeads): immobilization, hyperactivation and stabilization of the open form of lipases JM Palomo, G Muñoz, G Fernández-Lorente, C Mateo, ... Journal of Molecular Catalysis B: Enzymatic 19, 279-286, 2002 | 488 | 2002 |
Immobilization of lipases on hydrophobic supports: immobilization mechanism, advantages, problems, and solutions RC Rodrigues, JJ Virgen-Ortíz, JCS Dos Santos, Á Berenguer-Murcia, ... Biotechnology advances 37 (5), 746-770, 2019 | 484 | 2019 |
Glyoxyl agarose: a fully inert and hydrophilic support for immobilization and high stabilization of proteins C Mateo, JM Palomo, M Fuentes, L Betancor, V Grazu, F López-Gallego, ... Enzyme and Microbial Technology 39 (2), 274-280, 2006 | 454 | 2006 |
Increase in conformational stability of enzymes immobilized on epoxy-activated supports by favoring additional multipoint covalent attachment☆ C Mateo, O Abian, R Fernandez–Lafuente, JM Guisan Enzyme and Microbial Technology 26 (7), 509-515, 2000 | 426 | 2000 |
Epoxy sepabeads: a novel epoxy support for stabilization of industrial enzymes via very intense multipoint covalent attachment C Mateo, O Abian, G Fernández‐Lorente, J Pedroche, ... Biotechnology progress 18 (3), 629-634, 2002 | 407 | 2002 |