作者
Hui Li, Yi Yang, Ruifen Zhang, Yupin Cai, Xi Yang, Zhiyun Wang, Yuan Li, Xuemei Cheng, Xin Ye, Yang Xiang, Baoli Zhu
发表日期
2013/4/1
期刊
Journal of Clinical Virology
卷号
56
期号
4
页码范围
342-347
出版商
Elsevier
简介
BACKGROUND
Persistent infection with high-risk human papillomavirus (HPV) is necessary to cause cervical cancer, and integrating viral DNA into the host genome may contribute to the process of carcinogenesis. The underlying mechanisms are still unclear.
OBJECTIVE
In this study, we aimed to investigate the distribution of HPV 16 integration in the host genome and disrupted sites in the viral genome.
STUDY DESIGN
The physical status of HPV 16 genomes in 46 cervical precancerous and cancerous lesions was determined via ligation-mediated chain reaction (DIPS) using 15 previously published primer sets and 12 newly designed primer sets.
RESULTS
A total of 60 viral-cellular junctions were identified in 31 of 46 specimens, and over 80% of the integration sites in the human genome were located in regions of repetitive elements. The proportion of LSIL-, HSIL-, and SCC-containing integration sites near …
学术搜索中的文章
Preferential sites for the integration and disruption of human papillomavirus 16 in cervical lesions
H Li, Y Yang, R Zhang, Y Cai, X Yang, Z Wang, Y Li… - Journal of Clinical Virology, 2013