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Industrial automation and control systems (IACS) are tremendously employing supervisory control and data acquisition (SCADA) network. However, their integration into IACS is vulnerable to various cyber-attacks. In this article, we first present population extremal optimization (PEO)-based deep belief network detection method (PEO-DBN) to detect the cyber-attacks of SCADA-based IACS. In PEO-DBN method, PEO algorithm is employed to determine the DBN's parameters, including number of hidden units and the size of mini-batch and learning rate, as there is no clear knowledge to set these parameters. Then, to enhance the performance of single method for cyber-attacks detection, the ensemble learning scheme is introduced for aggregation of the proposed PEO-DBN method, called EnPEO-DBN. The proposed detection methods are evaluated on gas pipeline system dataset and water storage tank system …