Single‐Cell RNA Sequencing Analysis of the Drosophila Larval Ventral Cord

TH Nguyen, R Vicidomini, SD Choudhury… - Current …, 2021 - Wiley Online Library
Drosophila provides a powerful genetic system and an excellent model to study the
development and function of the nervous system. The fly's small brain and complex behavior …

Assembly and Exploration of a Single Cell Atlas of the Drosophila Larval Ventral Cord. Identification of Rare Cell Types

R Vicidomini, TH Nguyen, SD Choudhury… - Current …, 2021 - Wiley Online Library
Single‐cell RNA sequencing provides a new approach to an old problem: how to study
cellular diversity in complex biological systems. This powerful tool has been instrumental in …

Single‐cell RNA sequencing in Drosophila: Technologies and applications

H Li - Wiley Interdisciplinary Reviews: Developmental …, 2021 - Wiley Online Library
Single‐cell RNA sequencing (scRNA‐seq) has emerged as a powerful tool for investigating
cell states and functions at the single‐cell level. It has greatly revolutionized transcriptomic …

[HTML][HTML] DRscDB: A single-cell RNA-seq resource for data mining and data comparison across species

Y Hu, SG Tattikota, Y Liu, A Comjean, Y Gao… - Computational and …, 2021 - Elsevier
With the advent of single-cell RNA sequencing (scRNA-seq) technologies, there has been a
spike in studies involving scRNA-seq of several tissues across diverse species including …

Combined in Situ Hybridization Chain Reaction and Immunostaining to Visualize Gene Expression in Whole-Mount Drosophila Central Nervous Systems

JC Duckhorn, IP Junker, Y Ding, TR Shirangi - Behavioral Neurogenetics, 2022 - Springer
Methods to visualize gene expression in the Drosophila central nervous system are
important in fly neurogenetic studies. In this chapter, we describe a detailed protocol that …

scRNA-seq data from the larval Drosophila ventral cord provides a resource for studying motor systems function and development

TH Nguyen, R Vicidomini, SD Choudhury, TH Han… - Developmental Cell, 2024 - cell.com
The Drosophila larval ventral nerve cord (VNC) shares many similarities with the spinal cord
of vertebrates and has emerged as a major model for understanding the development and …

[HTML][HTML] A single-cell transcriptomic atlas of the adult Drosophila ventral nerve cord

AM Allen, MC Neville, S Birtles, V Croset, CD Treiber… - Elife, 2020 - elifesciences.org
The Drosophila ventral nerve cord (VNC) receives and processes descending signals from
the brain to produce a variety of coordinated locomotor outputs. It also integrates sensory …

A single-cell transcriptomic atlas of complete insect nervous systems across multiple life stages

M Corrales, BT Cocanougher, AB Kohn… - Neural …, 2022 - Springer
Molecular profiles of neurons influence neural development and function but bridging the
gap between genes, circuits, and behavior has been very difficult. Here we used single cell …

[HTML][HTML] Isolation and RNA sequencing of single nuclei from Drosophila tissues

CN McLaughlin, Y Qi, SR Quake, L Luo, H Li - STAR protocols, 2022 - Elsevier
Many insect cells are encapsulated within the exoskeleton and cannot be dissociated intact,
making them inaccessible to single-cell transcriptomic profiling. We have used single …

Methods and tools for spatial mapping of single-cell RNAseq clusters in Drosophila

SE Mohr, SG Tattikota, J Xu, J Zirin, Y Hu, N Perrimon - Genetics, 2021 - academic.oup.com
Single-cell RNA sequencing (scRNAseq) experiments provide a powerful means to identify
clusters of cells that share common gene expression signatures. A major challenge in …