By the onset of morphogenesis, Drosophila embryos consist of about 6000 cells that express
distinct gene combinations. Here, we used single-cell sequencing of precisely staged …

Drosophila melanogaster is a powerful, long-standing model for metazoan development and
gene regulation. We profiled chromatin accessibility in almost 1 million and gene expression …

INTRODUCTION Metazoan development represents a big jump in complexity compared
with unicellular life in two aspects: cell-type differentiation and cell spatial organization. In …

Drosophila has long been a successful model organism in multiple biomedical fields. Spatial
gene expression patterns are critical for the understanding of complex pathways and …

Background Cell-fate specification and tissue differentiation during development are largely
achieved by the regulation of gene transcription. Results As a first step to creating a …

INTRODUCTION During development, a single-cell zygote undergoes repeated cell
divisions to produce an embryo that contains many distinct cell types. This sequence of cell …

High-throughput mapping of cellular differentiation hierarchies from single-cell data
promises to empower systematic interrogations of vertebrate development and disease …

Background Cell and tissue specific gene expression is a defining feature of embryonic
development in multi-cellular organisms. However, the range of gene expression patterns …

Spatiotemporal orchestration of gene expression is required for proper embryonic
development. The use of single-cell technologies has begun to provide improved resolution …

Background To accurately describe gene expression and computationally model animal
transcriptional networks, it is essential to determine the changing locations of cells in …