Methods and tools for spatial mapping of single-cell RNAseq clusters in Drosophila

SE Mohr, SG Tattikota, J Xu, J Zirin, Y Hu, N Perrimon - Genetics, 2021 - academic.oup.com
Single-cell RNA sequencing (scRNAseq) experiments provide a powerful means to identify
clusters of cells that share common gene expression signatures. A major challenge in …

Single‐cell RNA sequencing in Drosophila: Technologies and applications

H Li - Wiley Interdisciplinary Reviews: Developmental …, 2021 - Wiley Online Library
Single‐cell RNA sequencing (scRNA‐seq) has emerged as a powerful tool for investigating
cell states and functions at the single‐cell level. It has greatly revolutionized transcriptomic …

Predicting cellular position in the Drosophila embryo from Single-Cell Transcriptomics data

J Tanevski, T Nguyen, B Truong, N Karaiskos… - bioRxiv, 2019 - biorxiv.org
Single-cell RNA-seq technologies are rapidly evolving but while very informative, in
standard scRNAseq experiments the spatial organization of the cells in the tissue of origin is …

[HTML][HTML] DRscDB: A single-cell RNA-seq resource for data mining and data comparison across species

Y Hu, SG Tattikota, Y Liu, A Comjean, Y Gao… - Computational and …, 2021 - Elsevier
With the advent of single-cell RNA sequencing (scRNA-seq) technologies, there has been a
spike in studies involving scRNA-seq of several tissues across diverse species including …

Gene expression analysis of sorted cells by RNA-seq in Drosophila intestine

J Chen, J Li, H Huang, R Xi - Bio-protocol, 2016 - bio-protocol.org
RNA sequencing (RNA-seq) has become a popular method for profiling gene expression.
Among many applications, one common purpose is to identify differentially expressed genes …

[HTML][HTML] Isolation and RNA sequencing of single nuclei from Drosophila tissues

CN McLaughlin, Y Qi, SR Quake, L Luo, H Li - STAR protocols, 2022 - Elsevier
Many insect cells are encapsulated within the exoskeleton and cannot be dissociated intact,
making them inaccessible to single-cell transcriptomic profiling. We have used single …

Assembly and Exploration of a Single Cell Atlas of the Drosophila Larval Ventral Cord. Identification of Rare Cell Types

R Vicidomini, TH Nguyen, SD Choudhury… - Current …, 2021 - Wiley Online Library
Single‐cell RNA sequencing provides a new approach to an old problem: how to study
cellular diversity in complex biological systems. This powerful tool has been instrumental in …

Single‐Cell RNA Sequencing Analysis of the Drosophila Larval Ventral Cord

TH Nguyen, R Vicidomini, SD Choudhury… - Current …, 2021 - Wiley Online Library
Drosophila provides a powerful genetic system and an excellent model to study the
development and function of the nervous system. The fly's small brain and complex behavior …

Gene selection for optimal prediction of cell position in tissues from single-cell transcriptomics data

J Tanevski, T Nguyen, B Truong… - Life Science …, 2020 - life-science-alliance.org
Single-cell RNA-sequencing (scRNAseq) technologies are rapidly evolving. Although very
informative, in standard scRNAseq experiments, the spatial organization of the cells in the …

Insect Insights at the Single-Cell Level: Technologies and Applications

C Sun, Y Shao, J Iqbal - Cells, 2023 - mdpi.com
Single-cell techniques are a promising way to unravel the complexity and heterogeneity of
transcripts at the cellular level and to reveal the composition of different cell types and …