Lessons from single cell sequencing in CNS cell specification and function

Z Li, WA Tyler, TF Haydar - Current opinion in genetics & development, 2020 - Elsevier
Modern RNA sequencing methods have greatly increased our understanding of the
molecular fingerprint of neurons, astrocytes and oligodendrocytes throughout the central …

Revealing cellular and molecular complexity of the central nervous system using single cell sequencing

Z Zeng, N Miao, T Sun - Stem cell research & therapy, 2018 - Springer
The mammalian central nervous system (CNS) is one of the most complex systems, with
thousands of cell types and subtypes with distinct and unique morphology and gene …

Cerebral cortical neuron diversity and development at single-cell resolution

MB Johnson, CA Walsh - Current opinion in neurobiology, 2017 - Elsevier
Highlights•Single-cell RNA sequencing revolutionizes systematic classification of neurons.•
Classification by scRNA-seq reveals new neuronal subtypes.•Matching transcriptionally and …

Beyond bulk: a review of single cell transcriptomics methodologies and applications

A Kulkarni, AG Anderson, DP Merullo… - Current opinion in …, 2019 - Elsevier
Highlights•Technologies such as single-cell RNA sequencing have rapidly progressed in
recent years.•Various methods exist for preparing libraries for sequencing, each with its own …

Significant evolutionary constraints on neuron cells revealed by single-cell transcriptomics

G Hu, J Li, GZ Wang - Genome Biology and Evolution, 2020 - academic.oup.com
Recent advances in single-cell RNA-sequencing technology have enabled us to
characterize a variety of different cell types in each brain region. However, the evolutionary …

Single-cell RNA sequencing: unraveling the brain one cell at a time

D Ofengeim, N Giagtzoglou, D Huh, C Zou… - Trends in molecular …, 2017 - cell.com
Single-cell RNA sequencing (scRNA-seq) is an exciting new technology allowing the
analysis of transcriptomes from individual cells, and is ideally suited to address the inherent …

Single-cell RNA-sequencing of the brain

R Cuevas-Diaz Duran, H Wei, JQ Wu - Clinical and translational medicine, 2017 - Springer
Single-cell RNA-sequencing (scRNA-seq) is revolutionizing our understanding of the
genomic, transcriptomic and epigenomic landscapes of cells within organs. The mammalian …

Single-cell sequencing of brain cell transcriptomes and epigenomes

EJ Armand, J Li, F Xie, C Luo, EA Mukamel - Neuron, 2021 - cell.com
Single-cell sequencing technologies, including transcriptomic and epigenomic assays, are
transforming our understanding of the cellular building blocks of neural circuits. By directly …

ShinyCortex: exploring single-cell transcriptome data from the developing human cortex

J Kageyama, D Wollny, B Treutlein… - Frontiers in …, 2018 - frontiersin.org
Single-cell mRNA sequencing (scRNA-seq) is a powerful method to identify and classify cell
types and reconstruct differentiation trajectories within complex tissues, such as the …

Targeted single-cell RNA sequencing of transcription factors enhances the identification of cell types and trajectories

A Pokhilko, AE Handel, F Curion, V Volpato… - Genome …, 2021 - genome.cshlp.org
Single-cell RNA sequencing (scRNA-seq) is a widely used method for identifying cell types
and trajectories in biologically heterogeneous samples, but it is limited in its detection and …