Multiplexed imaging of nucleome architectures in single cells of mammalian tissue
The three-dimensional architecture of the genome affects genomic functions. Multiple
genome architectures at different length scales, including chromatin loops, domains …
genome architectures at different length scales, including chromatin loops, domains …
Compartmentalization with nuclear landmarks yields random, yet precise, genome organization
The 3D organization of eukaryotic genomes plays an important role in genome function.
While significant progress has been made in deciphering the folding mechanisms of …
While significant progress has been made in deciphering the folding mechanisms of …
[HTML][HTML] Genome organization in the nucleus: from dynamic measurements to a functional model
A biological system is by definition a dynamic environment encompassing kinetic processes
that occur at different length scales and time ranges. To explore this type of system, spatial …
that occur at different length scales and time ranges. To explore this type of system, spatial …
Microscopy-based chromosome conformation capture enables simultaneous visualization of genome organization and transcription in intact organisms
Eukaryotic chromosomes are organized in multiple scales, from nucleosomes to
chromosome territories. Recently, genome-wide methods identified an intermediate level of …
chromosome territories. Recently, genome-wide methods identified an intermediate level of …
Visualization of loop extrusion by DNA nanoscale tracing in single human cells
KS Beckwith, Ø Ødegård-Fougner, NR Morero… - BioRxiv, 2021 - biorxiv.org
The spatial organization of the genome is essential for its functions, including gene
expression, DNA replication and repair, as well as chromosome segregation. Biomolecular …
expression, DNA replication and repair, as well as chromosome segregation. Biomolecular …
Chromatin tracing and multiplexed imaging of nucleome architectures (MINA) and RNAs in single mammalian cells and tissue
The genome is hierarchically organized into several 3D architectures, including chromatin
loops, domains, compartments and regions associated with nuclear lamina and nucleoli …
loops, domains, compartments and regions associated with nuclear lamina and nucleoli …
Super‐resolution microscopy of genome organization
SH Shim - Genes & genomics, 2021 - Springer
Recent advancements in sequencing and imaging technologies are providing new
perspectives in solving the mystery of three-dimensional folding of genome in a nucleus …
perspectives in solving the mystery of three-dimensional folding of genome in a nucleus …
Navigating the crowd: visualizing coordination between genome dynamics, structure, and transcription
The eukaryotic genome is hierarchically structured yet highly dynamic. Regulating
transcription in this environment demands a high level of coordination to permit many …
transcription in this environment demands a high level of coordination to permit many …
Hi-C: a method to study the three-dimensional architecture of genomes.
NL Van Berkum, E Lieberman-Aiden, L Williams… - JoVE (Journal of …, 2010 - jove.com
The three-dimensional folding of chromosomes compartmentalizes the genome and and
can bring distant functional elements, such as promoters and enhancers, into close spatial …
can bring distant functional elements, such as promoters and enhancers, into close spatial …
The second decade of 3C technologies: detailed insights into nuclear organization
A Denker, W De Laat - Genes & development, 2016 - genesdev.cshlp.org
The relevance of three-dimensional (3D) genome organization for transcriptional regulation
and thereby for cellular fate at large is now widely accepted. Our understanding of the …
and thereby for cellular fate at large is now widely accepted. Our understanding of the …