Recovery and analysis of transcriptome subsets from pooled single-cell RNA-seq libraries

KA Riemondy, M Ransom, C Alderman… - Nucleic acids …, 2019 - academic.oup.com
Single-cell RNA sequencing (scRNA-seq) methods generate sparse gene expression
profiles for thousands of single cells in a single experiment. The information in these profiles …

Introduction to single‐cell RNA sequencing

TK Olsen, N Baryawno - Current protocols in molecular biology, 2018 - Wiley Online Library
During the last decade, high‐throughput sequencing methods have revolutionized the entire
field of biology. The opportunity to study entire transcriptomes in great detail using RNA …

Scalable single-cell RNA sequencing from full transcripts with Smart-seq3xpress

M Hagemann-Jensen, C Ziegenhain… - Nature …, 2022 - nature.com
Current single-cell RNA sequencing (scRNA-seq) methods with high cellular throughputs
sacrifice full-transcript coverage and often sensitivity. Here we describe Smart-seq3xpress …

A scalable strand-specific protocol enabling full-length total RNA sequencing from single cells

S Haile, RD Corbett, VG LeBlanc, L Wei… - Frontiers in …, 2021 - frontiersin.org
RNA sequencing (RNAseq) has been widely used to generate bulk gene expression
measurements collected from pools of cells. Only relatively recently have single-cell …

Full-length single-cell RNA sequencing with smart-seq2

S Picelli - Single Cell Methods: Sequencing and Proteomics, 2019 - Springer
In the last few years single-cell RNA sequencing (scRNA-seq) has enabled the investigation
of cellular heterogeneity at the transcriptional level, the characterization of rare cell types as …

Tutorial: guidelines for the computational analysis of single-cell RNA sequencing data

TS Andrews, VY Kiselev, D McCarthy, M Hemberg - Nature protocols, 2021 - nature.com
Single-cell RNA sequencing (scRNA-seq) is a popular and powerful technology that allows
you to profile the whole transcriptome of a large number of individual cells. However, the …

Sensitive and powerful single-cell RNA sequencing using mcSCRB-seq

JW Bagnoli, C Ziegenhain, A Janjic, LE Wange… - Nature …, 2018 - nature.com
Single-cell RNA sequencing (scRNA-seq) has emerged as a central genome-wide method
to characterize cellular identities and processes. Consequently, improving its sensitivity …

Single-cell RNA sequencing technologies and bioinformatics pipelines

B Hwang, JH Lee, D Bang - Experimental & molecular medicine, 2018 - nature.com
Rapid progress in the development of next-generation sequencing (NGS) technologies in
recent years has provided many valuable insights into complex biological systems, ranging …

Comparative analysis of single-cell RNA sequencing methods with and without sample multiplexing

Y Xie, H Chen, VR Chellamuthu, AM Lajam… - International Journal of …, 2024 - mdpi.com
Single-cell RNA sequencing (scRNA-seq) has emerged as a powerful technique for
investigating biological heterogeneity at the single-cell level in human systems and model …

Comparative analysis of sequencing technologies for single-cell transcriptomics

KN Natarajan, Z Miao, M Jiang, X Huang, H Zhou, J Xie… - Genome Biology, 2019 - Springer
Single-cell RNA-seq technologies require library preparation prior to sequencing. Here, we
present the first report to compare the cheaper BGISEQ-500 platform to the Illumina HiSeq …