Insect Insights at the Single-Cell Level: Technologies and Applications

C Sun, Y Shao, J Iqbal - Cells, 2023 - mdpi.com
Single-cell techniques are a promising way to unravel the complexity and heterogeneity of
transcripts at the cellular level and to reveal the composition of different cell types and …

Single‐cell RNA sequencing in Drosophila: Technologies and applications

H Li - Wiley Interdisciplinary Reviews: Developmental …, 2021 - Wiley Online Library
Single‐cell RNA sequencing (scRNA‐seq) has emerged as a powerful tool for investigating
cell states and functions at the single‐cell level. It has greatly revolutionized transcriptomic …

[HTML][HTML] DRscDB: A single-cell RNA-seq resource for data mining and data comparison across species

Y Hu, SG Tattikota, Y Liu, A Comjean, Y Gao… - Computational and …, 2021 - Elsevier
With the advent of single-cell RNA sequencing (scRNA-seq) technologies, there has been a
spike in studies involving scRNA-seq of several tissues across diverse species including …

[HTML][HTML] Isolation and RNA sequencing of single nuclei from Drosophila tissues

CN McLaughlin, Y Qi, SR Quake, L Luo, H Li - STAR protocols, 2022 - Elsevier
Many insect cells are encapsulated within the exoskeleton and cannot be dissociated intact,
making them inaccessible to single-cell transcriptomic profiling. We have used single …

Analysis of Single-Cell Transcriptome Data in Drosophila

S Yousefian, MJ Musillo, J Bageritz - Drosophila: Methods and Protocols, 2022 - Springer
The fly Drosophila is a versatile model organism that has led to fascinating biological
discoveries. In the past few years, Drosophila researchers have used single-cell RNA …

Preparation of Drosophila larval blood cells for single-cell RNA sequencing

SG Tattikota, N Perrimon - Bio-protocol, 2021 - bio-protocol.org
Recent advances in single-cell RNA-sequencing (scRNA-seq) technologies provide
unprecedented opportunities to identify new cell types and characterize cell states. One of …

Methods and tools for spatial mapping of single-cell RNAseq clusters in Drosophila

SE Mohr, SG Tattikota, J Xu, J Zirin, Y Hu, N Perrimon - Genetics, 2021 - academic.oup.com
Single-cell RNA sequencing (scRNAseq) experiments provide a powerful means to identify
clusters of cells that share common gene expression signatures. A major challenge in …

Single‐cell RNA sequencing technologies and applications: A brief overview

D Jovic, X Liang, H Zeng, L Lin, F Xu… - Clinical and …, 2022 - Wiley Online Library
Single‐cell RNA sequencing (scRNA‐seq) technology has become the state‐of‐the‐art
approach for unravelling the heterogeneity and complexity of RNA transcripts within …

Single‐Cell RNA Sequencing Analysis of the Drosophila Larval Ventral Cord

TH Nguyen, R Vicidomini, SD Choudhury… - Current …, 2021 - Wiley Online Library
Drosophila provides a powerful genetic system and an excellent model to study the
development and function of the nervous system. The fly's small brain and complex behavior …

The evolution of single-cell RNA sequencing technology and application: progress and perspectives

S Wang, ST Sun, XY Zhang, HR Ding, Y Yuan… - International Journal of …, 2023 - mdpi.com
As an emerging sequencing technology, single-cell RNA sequencing (scRNA-Seq) has
become a powerful tool for describing cell subpopulation classification and cell …