Introduction to single‐cell RNA sequencing

TK Olsen, N Baryawno - Current protocols in molecular biology, 2018 - Wiley Online Library
During the last decade, high‐throughput sequencing methods have revolutionized the entire
field of biology. The opportunity to study entire transcriptomes in great detail using RNA …

Single-cell RNA sequencing analysis: a step-by-step overview

S Slovin, A Carissimo, F Panariello, A Grimaldi… - RNA bioinformatics, 2021 - Springer
Thanks to innovative sample-preparation and sequencing technologies, gene expression in
individual cells can now be measured for thousands of cells in a single experiment. Since its …

Methods, challenges and potentials of single cell RNA-seq

D Hebenstreit - Biology, 2012 - mdpi.com
RNA-sequencing (RNA-seq) has become the tool of choice for transcriptomics. Several
recent studies demonstrate its successful adaption to single cell analysis. This allows new …

Tutorial: guidelines for the computational analysis of single-cell RNA sequencing data

TS Andrews, VY Kiselev, D McCarthy, M Hemberg - Nature protocols, 2021 - nature.com
Single-cell RNA sequencing (scRNA-seq) is a popular and powerful technology that allows
you to profile the whole transcriptome of a large number of individual cells. However, the …

Quantitative single-cell transcriptomics

C Ziegenhain, B Vieth, S Parekh… - Briefings in …, 2018 - academic.oup.com
Single-cell RNA sequencing (scRNA-seq) is currently transforming our understanding of
biology, as it is a powerful tool to resolve cellular heterogeneity and molecular networks …

Single-cell RNA-seq technologies and related computational data analysis

G Chen, B Ning, T Shi - Frontiers in genetics, 2019 - frontiersin.org
Single-cell RNA sequencing (scRNA-seq) technologies allow the dissection of gene
expression at single-cell resolution, which greatly revolutionizes transcriptomic studies. A …

A practical guide to single-cell RNA-sequencing for biomedical research and clinical applications

A Haque, J Engel, SA Teichmann, T Lönnberg - Genome medicine, 2017 - Springer
RNA sequencing (RNA-seq) is a genomic approach for the detection and quantitative
analysis of messenger RNA molecules in a biological sample and is useful for studying …

Single-cell RNA sequencing and its combination with protein and DNA analyses

JR Choi, KW Yong, JY Choi, AC Cowie - Cells, 2020 - mdpi.com
Heterogeneity in cell populations poses a significant challenge for understanding complex
cell biological processes. The analysis of cells at the single-cell level, especially single-cell …

scRNASeqDB: a database for RNA-Seq based gene expression profiles in human single cells

Y Cao, J Zhu, P Jia, Z Zhao - Genes, 2017 - mdpi.com
Single-cell RNA sequencing (scRNA-Seq) is rapidly becoming a powerful tool for high-
throughput transcriptomic analysis of cell states and dynamics at the single cell level. Both …

Full-length single-cell RNA sequencing with smart-seq2

S Picelli - Single Cell Methods: Sequencing and Proteomics, 2019 - Springer
In the last few years single-cell RNA sequencing (scRNA-seq) has enabled the investigation
of cellular heterogeneity at the transcriptional level, the characterization of rare cell types as …