Maintenance of age in human neurons generated by microRNA-based neuronal conversion of fibroblasts

CJ Huh, B Zhang, MB Victor, S Dahiya, LFZ Batista… - elife, 2016 - elifesciences.org
Aging is a major risk factor in many forms of late-onset neurodegenerative disorders. The
ability to recapitulate age-related characteristics of human neurons in culture will offer …

Directly reprogrammed human neurons retain aging-associated transcriptomic signatures and reveal age-related nucleocytoplasmic defects

J Mertens, ACM Paquola, M Ku, E Hatch, L Böhnke… - Cell stem cell, 2015 - cell.com
Aging is a major risk factor for many human diseases, and in vitro generation of human
neurons is an attractive approach for modeling aging-related brain disorders. However …

Aging in a dish: iPSC-derived and directly induced neurons for studying brain aging and age-related neurodegenerative diseases

J Mertens, D Reid, S Lau, Y Kim… - Annual review of …, 2018 - annualreviews.org
Age-associated neurological diseases represent a profound challenge in biomedical
research as we are still struggling to understand the interface between the aging process …

Age-dependent instability of mature neuronal fate in induced neurons from Alzheimer's patients

J Mertens, JR Herdy, L Traxler, ST Schafer… - Cell stem cell, 2021 - cell.com
Sporadic Alzheimer's disease (AD) exclusively affects elderly people. Using direct
conversion of AD patient fibroblasts into induced neurons (iNs), we generated an age …

Cell-type-specific aging clocks to quantify aging and rejuvenation in neurogenic regions of the brain

MT Buckley, ED Sun, BM George, L Liu, N Schaum… - Nature Aging, 2023 - nature.com
The diversity of cell types is a challenge for quantifying aging and its reversal. Here we
develop 'aging clocks' based on single-cell transcriptomics to characterize cell-type-specific …

A stably self-renewing adult blood-derived induced neural stem cell exhibiting patternability and epigenetic rejuvenation

C Sheng, J Jungverdorben, H Wiethoff, Q Lin… - Nature …, 2018 - nature.com
Recent reports suggest that induced neurons (iNs), but not induced pluripotent stem cell
(iPSC)-derived neurons, largely preserve age-associated traits. Here, we report on the …

Direct reprogramming rather than iPSC-based reprogramming maintains aging hallmarks in human motor neurons

Y Tang, ML Liu, T Zang, CL Zhang - Frontiers in molecular …, 2017 - frontiersin.org
In vitro generation of motor neurons (MNs) is a promising approach for modeling motor
neuron diseases (MNDs) such as amyotrophic lateral sclerosis (ALS). As aging is a leading …

Next‐generation disease modeling with direct conversion: a new path to old neurons

L Traxler, F Edenhofer, J Mertens - FEBS letters, 2019 - Wiley Online Library
Within just over a decade, human reprogramming‐based disease modeling has developed
from a rather outlandish idea into an essential part of disease research. While iPSCs are a …

Multi-omic rejuvenation of human cells by maturation phase transient reprogramming

D Gill, A Parry, F Santos, H Okkenhaug, CD Todd… - Elife, 2022 - elifesciences.org
Ageing is the gradual decline in organismal fitness that occurs over time leading to tissue
dysfunction and disease. At the cellular level, ageing is associated with reduced function …

Targeted transgene expression in neuronal precursors: watching young neurons in the old brain

S Couillard‐Despres, B Winner, C Karl… - European Journal of …, 2006 - Wiley Online Library
Progress in the field of neurogenesis is limited by the lack of animal models allowing direct
detection and analysis of living cells participating in neurogenesis. We engineered a …