Generalizing RNA velocity to transient cell states through dynamical modeling

V Bergen, M Lange, S Peidli, FA Wolf, FJ Theis - Nature biotechnology, 2020 - nature.com
RNA velocity has opened up new ways of studying cellular differentiation in single-cell RNA-…
based on the ratio of its spliced and unspliced messenger RNA (mRNA). However, errors in …

RNA velocity unraveled

G Gorin, M Fang, T Chari, L Pachter - PLOS Computational …, 2022 - journals.plos.org
… We perform a thorough analysis of RNA velocity methods, with … Finally, we argue for a more
rigorous approach to RNA velocity, … Our findings on RNA velocity have implications for other …

Dynamical systems model of RNA velocity improves inference of single-cell trajectory, pseudo-time and gene regulation

R Liu, AO Pisco, E Braun, S Linnarsson… - Journal of Molecular …, 2022 - Elsevier
… been made to incorporate RNA velocity, such as projecting RNA velocity into 2D embeddings
to … based on RNA velocity 14 and constructing nearest neighbor graph with RNA velocity. …

Deep generative modeling of transcriptional dynamics for RNA velocity analysis in single cells

A Gayoso, P Weiler, M Lotfollahi, D Klein, J Hong… - Nature …, 2024 - nature.com
… We begin with the formulation of the ‘dynamical’ model of RNA velocity as presented by
ref. . We posit transcriptional states k ∈ {1, 2, 3, 4}, where k = 1 indicates induction, k = 2 …

RNA velocity—current challenges and future perspectives

V Bergen, RA Soldatov, PV Kharchenko… - Molecular systems …, 2021 - embopress.org
… cellular dynamics. Here, we review the current state of RNA velocity modeling approaches,
… We then outline future directions on how to generalize the concept of RNA velocity to a …

RNA velocity of single cells

G La Manno, R Soldatov, A Zeisel, E Braun… - Nature, 2018 - nature.com
… Here we show that RNA velocity—the time derivative of the gene expression state—can be
… in common single-cell RNA sequencing protocols. RNA velocity is a high-dimensional vector …

On the mathematics of RNA velocity I: theoretical analysis

T Li, J Shi, Y Wu, P Zhou - bioRxiv, 2020 - biorxiv.org
… In Section 3 we will revisit the existing algorithms to infer parameters in RNA velocity models
models. In Section 4 we focus on the dynamical system analysis based on RNA velocity, …

Pumping the brakes on RNA velocity by understanding and interpreting RNA velocity estimates

SC Zheng, G Stein-O'Brien, L Boukas, LA Goff… - Genome biology, 2023 - Springer
… Our findings emphasize the importance of choices in the RNA velocitydynamics using RNA
velocity, particularly in terms of speed. Finally, we emphasize that the use of RNA velocity in …

A relay velocity model infers cell-dependent RNA velocity

S Li, P Zhang, W Chen, L Ye, KW Brannan, NT Le… - Nature …, 2024 - nature.com
… We show that cellDancer overcomes the limitations of existing RNA velocity models in
modeling erythroid maturation and hippocampus development. Moreover, cellDancer provides cell…

A guide to trajectory inference and RNA velocity

P Weiler, K Van den Berge, K Street, S Tiberi - Single Cell Transcriptomics …, 2022 - Springer
… inference methods reconstruct cellular dynamics given a population of cells of varying maturity,
RNA velocity relies on a dynamical model describing splicing dynamics. Here, we initially …