Detection and analysis of circular RNAs by RT-PCR

AC Panda, M Gorospe - Bio-protocol, 2018 - bio-protocol.org
… not the linear RNAs with the same sequence. The PCR amplicon for the detection of circRNAs
using … This method has been successfully used in several studies for the detection and …

Comparison of real-time RT-PCR assays for hepatitis E virus RNA detection

C Mokhtari, E Marchadier, S Haïm-Boukobza… - Journal of Clinical …, 2013 - Elsevier
… five real-time PCR procedures for HEV RNA detection were … ) international standard for HEV
RNA should allow a more … of five different real time PCR methods targeting either ORF2/3 7 …

[HTML][HTML] Comprehensive molecular screening: from the RT-PCR to the RNA-seq

C Costa, A Giménez-Capitán… - … lung cancer research, 2013 - ncbi.nlm.nih.gov
reverse transcriptase enzyme led to the development of reverse transcription polymerase
chain reaction (RT-PCR) … blot as the method of choice for RNA detection and quantification (3). …

RNA diagnostics: realtime RTPCR strategies and promising novel target RNAs

G Mayer, J Müller, CE Lünse - … Interdisciplinary Reviews: RNA, 2011 - Wiley Online Library
… of some pathogens is stored as RNA, allowing their sensitive detection using nucleic acid …
of detecting different RNA types by real-time reverse-transcription polymerase chain reaction

PCR for detection of rubella virus RNA in clinical samples

TJ Bosma, KM Corbett, S O'Shea… - Journal of Clinical …, 1995 - Am Soc Microbiol
… and sensitive RT nested PCR assay (RT-PCR) for the detection of rubella virus RNA by
using … The sensitivity of our RT-PCR also compares favorably with an RTPCR for enterovirus …

Quantitative real-time RT-PCR–a perspective

SA Bustin, V Benes, T Nolan… - Journal of molecular …, 2005 - jme.bioscientifica.com
… steps into one homogeneous assay and obviates the need for gel electrophoresis to detect
real-time RT-PCR the benchmark technology for the detection and/or comparison of RNA

Development of a TaqMan® RT-PCR assay without RNA extraction step for the detection and quantification of African Chikungunya viruses

B Pastorino, M Bessaud, M Grandadam, S Murri… - Journal of virological …, 2005 - Elsevier
… In pilot experiments, the Platinium Quantitative RT-PCR Thermoscript One-Step System (…
in our laboratory for real-time RT-PCR detection of RNA viruses: the LightCycler-RNA Master …

Development of a real-time reverse-transcription PCR for detection of Newcastle disease virus RNA in clinical samples

MG Wise, DL Suarez, BS Seal… - Journal of clinical …, 2004 - Am Soc Microbiol
… in this investigation detected more samples and lower concentrations of RNA than the …
The objective of this study was to develop a rapid real-time RT-PCR for detection and …

Development of three multiplex RT-PCR assays for the detection of 12 respiratory RNA viruses

S Bellau-Pujol, A Vabret, L Legrand, J Dina… - Journal of virological …, 2005 - Elsevier
… Three multiplex hemi-nested RT-PCR assays were developed to detect simultaneously 12
RNART-PCR methods for the detection of RNA viruses involved in respiratory diseases. …

Real-time RT-PCR for quantitation of hepatitis C virus RNA

JH Yang, JP Lai, SD Douglas, D Metzger… - Journal of virological …, 2002 - Elsevier
… In addition, real-time PCR makes quantitation of DNA and RNA much more precise and …
2), we were able to detect HCV RNA as low as 100 copies per action with 60% detection