Field and in vitro assay of three methods for freezing ram semen
Glycerol has been the most widely used cryopreservation agent for spermatozoa and a wide
range of factors affect its action on sperm viability and fertilizing capacity. We tested three
methods for freezing ram semen packed in 0.25 ml straws (final cellular concentration: 100×
106spz/ml). Method M1: Two-thirds of the final volume of diluent was added as solution A
(without glycerol) to the pure semen at 35° C. The sample was cooled to 5° C (− 0.30°
C/min), one-third of final diluent volume was added as solution B (final concentration of …
range of factors affect its action on sperm viability and fertilizing capacity. We tested three
methods for freezing ram semen packed in 0.25 ml straws (final cellular concentration: 100×
106spz/ml). Method M1: Two-thirds of the final volume of diluent was added as solution A
(without glycerol) to the pure semen at 35° C. The sample was cooled to 5° C (− 0.30°
C/min), one-third of final diluent volume was added as solution B (final concentration of …
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