Escalating stress and pressures, along with organisational change in universities has led to the increased importance of research in to the impact of perceived job stress, work-life balance and work-life conflict amongst academics. Yet, very few studies have examined academics’ ability to balance work and personal life, and overcome work-life conflict. Drawing on Spillover theory (Zedeck, 1992), our study hypothesised that high levels of perceived job pressure stress and job threat stress would predict increased levels of work-life conflict, and decreased levels of work-life balance. Due to the well-documented relationship between stress and health, the influence of job stress on wellbeing was also investigated in this sample of academics (N= 139). Perceived job stress (threat and pressure-type stressors) was associated with poorer work-life balance, and increased conflict between academics’ work and personal lives. Perceived job threat-type stress made a stronger contribution and was a significant predictor of work-life balance and work-life conflict scores, than perceived job pressure-type stress. Perceived job threattype stress among academics was also a significant predictor and associated with poorer wellbeing and increased ill-being, but perceived job pressure-type stress was not related to academics’ wellbeing or ill-being.