Problems related to CPD preserved blood used for NEQAS trials in haematology
MHJ Leyssen, MJG De Bruyere… - Clinical & Laboratory …, 1985 - Wiley Online Library
MHJ Leyssen, MJG De Bruyere, VJM Van Duppen, RL Verwilghen
Clinical & Laboratory Haematology, 1985•Wiley Online LibraryA study of the results of the Belgian National External Quality Assessment Scheme (NEQAS)
in haematology, revealed marked differences in MCV values obtained with instruments of
different types. For this reason the citrate‐phosphate‐dextrose (CPD) preserved blood used
as NEQAS material was analysed daily with a Coulter S‐Plus IV and with a cross‐calibrated
Ortho ELT‐800. The observed differences in MCV are apparently due to differences in
measuring apparatus and also to the changing characteristics of the erythrocytes. Ageing of …
in haematology, revealed marked differences in MCV values obtained with instruments of
different types. For this reason the citrate‐phosphate‐dextrose (CPD) preserved blood used
as NEQAS material was analysed daily with a Coulter S‐Plus IV and with a cross‐calibrated
Ortho ELT‐800. The observed differences in MCV are apparently due to differences in
measuring apparatus and also to the changing characteristics of the erythrocytes. Ageing of …
Summary
A study of the results of the Belgian National External Quality Assessment Scheme (NEQAS) in haematology, revealed marked differences in MCV values obtained with instruments of different types. For this reason the citrate‐phosphate‐dextrose (CPD) preserved blood used as NEQAS material was analysed daily with a Coulter S‐Plus IV and with a cross‐calibrated Ortho ELT‐800. The observed differences in MCV are apparently due to differences in measuring apparatus and also to the changing characteristics of the erythrocytes. Ageing of CPD blood during 1 to 4 days (postal delivery) influenced markedly the MCV as measured with the Ortho ELT systems.
These time‐ and instrument‐related factors influence the measurements, they interfere with the statistical evaluation of NEQAS results and can lead to errors in the evaluation of individual performers.
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