Flow cytometry of extracellular vesicles: potential, pitfalls, and prospects

JP Nolan - Current protocols in cytometry, 2015 - Wiley Online Library
Evidence suggests that extracellular vesicles (EVs) can play roles in physiology and
pathology, providing impetus to explore their use as diagnostic and therapeutic targets …

Isolation of cancer-derived extracellular vesicle subpopulations by a size-selective microfluidic platform

Z Chen, Y Yang, H Yamaguchi, MC Hung… - …, 2020 - pubs.aip.org
Extracellular vesicles (EVs) play an important role in intercellular communication. Recently,
there has been increasing interest in EVs as potential diagnostic biomarkers and therapeutic …

Isolation of extracellular vesicles by ultracentrifugation

F Momen-Heravi - Extracellular Vesicles: Methods and Protocols, 2017 - Springer
Extracellular vesicles (EVs) represent a group of heterogeneous vesicles that can be
obtained from almost all biofluids. EVs, including microvesicles, exosomes, and apoptotic …

[HTML][HTML] The methods of choice for extracellular vesicles (EVs) characterization

R Szatanek, M Baj-Krzyworzeka, J Zimoch… - International journal of …, 2017 - mdpi.com
In recent years, extracellular vesicles (EVs) have become a subject of intense study. These
membrane-enclosed spherical structures are secreted by almost every cell type and are …

[HTML][HTML] Methods for separation and characterization of extracellular vesicles: results of a worldwide survey performed by the ISEV rigor and standardization …

F Royo, C Théry, JM Falcón-Pérez, R Nieuwland… - Cells, 2020 - mdpi.com
Research on extracellular vesicles (EVs) is growing exponentially due to an increasing
appreciation of EVs as disease biomarkers and therapeutics, an expanding number of EV …

Ultrasensitive single extracellular vesicle detection using high throughput droplet digital enzyme-linked immunosorbent assay

Z Yang, Y Atiyas, H Shen, MJ Siedlik, J Wu, K Beard… - Nano …, 2022 - ACS Publications
Extracellular vesicles (EVs) have attracted enormous attention for their diagnostic and
therapeutic potential. However, it has proven challenging to achieve the sensitivity to detect …

[HTML][HTML] An imaging flow cytometry-based methodology for the analysis of single extracellular vesicles in unprocessed human plasma

WW Woud, E van der Pol, E Mul, MJ Hoogduijn… - Communications …, 2022 - nature.com
Extracellular vesicles (EVs) are tissue-specific particles released by cells containing
valuable diagnostic information in the form of various biomolecules. To rule out selection …

[HTML][HTML] Recent advances in optical label-free characterization of extracellular vesicles

M Imanbekova, S Suarasan, Y Lu, S Jurchuk… - …, 2022 - degruyter.com
Extracellular vesicles (EVs) are complex biological nanoparticles endogenously secreted by
all eukaryotic cells. EVs carry a specific molecular cargo of proteins, lipids, and nucleic acids …

Advances in technologies for purification and enrichment of extracellular vesicles

P Zhang, JC Yeo, CT Lim - … TECHNOLOGY: Translating Life …, 2019 - journals.sagepub.com
Extracellular vesicles (EVs) are lipid bilayer-bound vesicles secreted by cells. Subtypes of
EVs such as microvesicles and exosomes are further categorized mainly by their different …

A protocol for isolation and proteomic characterization of distinct extracellular vesicle subtypes by sequential centrifugal ultrafiltration

R Xu, RJ Simpson, DW Greening - Exosomes and Microvesicles: Methods …, 2017 - Springer
Scientific and clinical interest in extracellular vesicles (EVs) has increased rapidly as
evidence mounts that they may constitute a new signaling paradigm. Recent studies have …