Analytical challenges of extracellular vesicle detection: A comparison of different techniques

U Erdbrügger, J Lannigan - Cytometry Part A, 2016 - Wiley Online Library
The interest in extracellular vesicles (EVs) has grown exponentially over the last decade.
Evolving evidence is demonstrating that these EVs are playing an important role in health …

[HTML][HTML] Toward characterizing extracellular vesicles at a single-particle level

C Chiang, C Chen - Journal of biomedical science, 2019 - Springer
Extracellular vesicles (EVs) are cell-derived membrane-bound vesicles that serve a means
of cell-cell communication. Studying EVs at a single-particle level is important because EVs …

[HTML][HTML] Alternative methods for characterization of extracellular vesicles

F Momen-Heravi, L Balaj, S Alian, J Tigges… - Frontiers in …, 2012 - frontiersin.org
Extracellular vesicles (ECVs) are nano-sized vesicles released by all cells in vitro as well as
in vivo. Their role has been implicated mainly in cell–cell communication, but also in disease …

[HTML][HTML] Methods for separation and characterization of extracellular vesicles: results of a worldwide survey performed by the ISEV rigor and standardization …

F Royo, C Théry, JM Falcón-Pérez, R Nieuwland… - Cells, 2020 - mdpi.com
Research on extracellular vesicles (EVs) is growing exponentially due to an increasing
appreciation of EVs as disease biomarkers and therapeutics, an expanding number of EV …

[HTML][HTML] Potential and challenges of specifically isolating extracellular vesicles from heterogeneous populations

S Allelein, P Medina-Perez, ALH Lopes, S Rau… - Scientific reports, 2021 - nature.com
Extracellular vesicles (EVs) have attracted interest due to their ability to provide diagnostic
information from liquid biopsies. Cells constantly release vesicles divers in size, content and …

[HTML][HTML] Particle size distribution of exosomes and microvesicles determined by transmission electron microscopy, flow cytometry, nanoparticle tracking analysis, and …

E Van der Pol, FAW Coumans, AE Grootemaat… - Journal of Thrombosis …, 2014 - Elsevier
Background Enumeration of extracellular vesicles has clinical potential as a biomarker for
disease. In biological samples, the smallest and largest vesicles typically differ 25‐fold in …

[HTML][HTML] A perspective on the isolation and characterization of extracellular vesicles from different biofluids

R Bano, F Ahmad, M Mohsin - RSC advances, 2021 - pubs.rsc.org
Extracellular vesicles (EVs) are small membrane-bound particles, which include exosomes,
micro vesicles (MVs) and various-sized vesicles, released by healthy and diseased cells …

Optimisation of imaging flow cytometry for the analysis of single extracellular vesicles by using fluorescence-tagged vesicles as biological reference material

A Görgens, M Bremer, R Ferrer-Tur… - Journal of …, 2019 - Taylor & Francis
Extracellular vesicles (EVs) mediate targeted cellular interactions in normal and
pathophysiological conditions and are increasingly recognised as potential biomarkers …

[HTML][HTML] A bead-assisted flow cytometry method for the semi-quantitative analysis of Extracellular Vesicles

H Suárez, A Gámez-Valero, R Reyes, S López-Martín… - Scientific reports, 2017 - nature.com
Most experimental approaches commonly employed for the characterization and
quantitation of EVs are time consuming, require of specialized instrumentation and often are …

Towards traceable size determination of extracellular vesicles

Z Varga, Y Yuana, AE Grootemaat… - Journal of …, 2014 - Taylor & Francis
Background Extracellular vesicles (EVs) have clinical importance due to their roles in a wide
range of biological processes. The detection and characterization of EVs are challenging …