Previous research is limited regarding factors influencing tram-involved serious injury crashes. The aim of this study is to identify key vehicle, road, environment and driver related factors associated with tram-involved serious injury crashes. Using a binary logistic regression modelling approach, the following factors were identified to be significant in influencing tram-involved fatal crashes in Melbourne: tram floor height, tram age, season, traffic volume, tram lane priority and tram travel speed. Low floor trams, older trams, tram priority lanes and higher tram travelling speeds are more likely to increase tram-involved fatal crashes. Higher traffic volume decreases the likelihood of serious crashes. Fatal crashes are more likely to occur during spring and summer. Findings from this study may offer ideas for future research in the area of tram safety and help to develop countermeasures to prevent specific fatality types from occurring.