Employing flow cytometry to extracellular vesicles sample microvolume analysis and quality control

J Maia, S Batista, N Couto, AC Gregório… - Frontiers in Cell and …, 2020 - frontiersin.org
… labeling with CFSE as a means for determining quality control of purification protocols of …
vesicular particles in biofluids, and thus the suitability of our FC strategy for quality control and …

Standardization of blood collection and processing for the diagnostic use of extracellular vesicles

M Venturella, FM Carpi, D Zocco - Current Pathobiology Reports, 2019 - Springer
… preanalytical variables that affect sample quality and suitability for EV-based diagnostic
approaches. Furthermore, we suggest biochemical and molecular quality control (QC) metrics to …

Methods for separation and characterization of extracellular vesicles: results of a worldwide survey performed by the ISEV rigor and standardization subcommittee

F Royo, C Théry, JM Falcón-Pérez, R Nieuwland… - Cells, 2020 - mdpi.com
… and quality controls are currently … Extracellular Vesicles (ISEV) performed a worldwide
survey in 2015 on “Techniques used for the isolation and characterization of extracellular vesicles

[HTML][HTML] Manufacturing and characterization of extracellular vesicles from umbilical cord–derived mesenchymal stromal cells for clinical testing

E Rohde, K Pachler, M Gimona - Cytotherapy, 2019 - Elsevier
quality control of umbilical cord–derived MSC-EVs. We introduce guidelines of good
manufacturing practice and their practicability along the path from the laboratory to the patient. We …

Mesenchymal stem cell-derived extracellular vesicles: Progress and remaining hurdles in developing regulatory compliant quality control assays

JKE Chua, J Lim, LH Foong, CY Mok, HY Tan… - Cell Biology and …, 2022 - Springer
quality control (QC) measurement. In particular, QC assays which are crucial to confirm the
safety, efficacy, and quality of … of MSC-EVs and quality control guidelines for biologics are …

Quality control of bacterial extracellular vesicles with total protein content assay, nanoparticles tracking analysis, and capillary electrophoresis

A Steć, J Jońca, K Waleron, M Waleron… - International Journal of …, 2022 - mdpi.com
Extracellular vesicles (EVs) were isolated from Pectobacterium … selectivity of CE in quality
control of EVs and its ability to … This makes the strict quality control of isolates an inherent part …

Fast, simple and calibration-free size characterization and quality control of extracellular vesicles using capillary Taylor dispersion analysis

S Obeid, J Chamieh, TD Mai, M Morani, M Reyre… - … of Chromatography A, 2023 - Elsevier
… Dispersion Analysis (TDA) method for the size characterization of Extracellular Vesicles (EVs),
which are highly heterogeneous nanoscale cell-derived vesicles (30–1000 nm). Here, we …

[HTML][HTML] Rigor and standardization of extracellular vesicle research: Paving the road towards robustness

R Nieuwland, JM Falcón‐Pérez, C Théry… - … Extracellular Vesicles, 2020 - ncbi.nlm.nih.gov
… , along with implementation of sample quality control measures. Interestingly, the results …
to quality control. A majority of these 'biobankers' indicated that they performed quality control of …

Considerations towards a roadmap for collection, handling and storage of blood extracellular vesicles

A Clayton, E Boilard, EI Buzas, L Cheng… - … extracellular vesicles, 2019 - Taylor & Francis
… From the answers it is clear that most respondents are in favour of evidence-based research,
education, quality control procedures, and physical models to improve our understanding …

Chromatographic approaches for purification and analytical characterization of extracellular vesicles: recent advancements

S Tengattini - Chromatographia, 2019 - Springer
Extracellular vesicles (EVs) are nanoscale particles released … a real standardization of
isolation and quality control procedures is far to … approaches in EV purification and quality control. …