[PDF][PDF] Practical uses on nanopore long-read sequencing in a high-throughput contract food microbiology laboratory

AA Benkowski - a.storyblok.com
… genome sequencing using ONT can allow strain-level identification and tracking of pathogens
or … monitored for pathogens using swabs and qualitative rapid pathogen testing. When …

Precision long-read metagenomics sequencing for food safety by detection and assembly of Shiga toxin-producing Escherichia coli in irrigation water

M Maguire, JA Kase, D Roberson, T Muruvanda… - PloS one, 2021 - journals.plos.org
foodborne illnesses and outbreak investigations [24]. WGS is being used to monitor and identify
foodborne pathogens [… and detection limit of nanopore sequencing on STEC enrichment …

Bacterial Metabarcoding of Raw Palm Sap Samples from Bangladesh with Nanopore Sequencing

Á Ábrahám, MN Islam, Z Gazdag, SA Khan… - Foods, 2024 - mdpi.com
… Since the dominance of either of these bacteria was obvious in the pre-market stage of
collection, we hypothesize the natural fermentation process as the protective state of this product, …

… wheat flour: detection and serotyping by a quasimetagenomic approach assisted by magnetic capture, multiple-displacement amplification, and real-time sequencing

F Forghani, S Li, S Zhang, DA Mann… - Applied and …, 2020 - Am Soc Microbiol
… can detect and subtype foodborne pathogens in a single … (MDA), and nanopore
sequencing real-time analysis for … 1 h of sequencing in a nanopore sequencer. Flour samples …

Application of whole genome sequencing (WGS) approach against identification of foodborne bacteria

S Bharadwaj, VD Dwivedi, N Kirtipal - Microbial Genomics in Sustainable …, 2019 - Springer
Foodborne pathogens mainly comprise the bacteria and fungi that have been frequently
reported for the foodborne illnesses through consumption of spoiled food as well as epidemic …

The present and future of whole genome sequencing (WGS) and whole metagenome sequencing (WMS) for surveillance of antimicrobial resistant microorganisms …

EA Oniciuc, E Likotrafiti, A Alvarez-Molina, M Prieto… - Genes, 2018 - mdpi.com
… resistant strains from various foodborne pathogens, such as … whole genome sequencing
(WGS) of bacterial pathogens for … or non-cultivable microorganisms, and therefore could assist …

Nanopore sequencing for fast determination of plasmids, phages, virulence markers, and antimicrobial resistance genes in Shiga toxin-producing Escherichia coli

N Gonzalez-Escalona, MA Allard, EW Brown… - PLoS …, 2019 - journals.plos.org
… a foodborne outbreak investigation, 2) important clues about the long-term evolution of enteric
pathogens, 3) … identification of viral pathogens in clinical samples by real-time nanopore

A review on the applications of next generation sequencing technologies as applied to food-related microbiome studies

Y Cao, S Fanning, S Proos, K Jordan… - Frontiers in …, 2017 - frontiersin.org
… the transmission of foodborne pathogens across international … as a single strand through
the nanopore. Base calling is then … 16S rDNA sequencing is the ability to characterize bacteria

… gap: Oxford Nanopore Technologies R10 sequencing allows comparable results to Illumina sequencing for SNP-based outbreak investigation of bacterial pathogens

B Bogaerts, A Van den Bossche… - Journal of Clinical …, 2024 - Am Soc Microbiol
… monocytogenes, 14 ST6 isolates were selected, six of which were associated with a
suspected foodborne outbreak that occurred in 2020. Routine surveillance using Illumina …

Whole-genome sequencing of Listeria monocytogenes isolated from the first listeriosis foodborne outbreak in South Korea

SH Lee, S Lee, SH Park, OK Koo - Frontiers in Microbiology, 2023 - frontiersin.org
Nanopore sequencing, a MinION sequencing library was prepared using the Nanopore
Ligation Sequencing … to inhibit protein synthesis in bacteria. Previous studies showed resistance …