Introduction Shotgun metagenomics has previously proven effective in the investigation of
foodborne outbreaks by providing rapid and comprehensive insights into the microbial …

The current routine laboratory practices to investigate food samples in case of foodborne
outbreaks still rely on attempts to isolate the pathogen in order to characterize it. We present …

Viable pathogenic bacteria are major biohazards that pose a significant threat to food safety.
Despite the recent developments in detection platforms, multiplex identification of viable …

The management of a foodborne outbreak depends on the rapid and accurate identification
of the responsible food source. Conventional methods based on isolation of the pathogen …

Rapid, accurate and comprehensive diagnostics are essential for outbreak prevention and
pathogen surveillance. Real-time, on-site metagenomics on miniaturized devices, such as …

Foodborne pathogens, particularly antimicrobial-resistant (AMR) bacteria, remain a
significant threat to global health. Given the limitations of conventional culture-based …

Culture-independent diagnostics, such as metagenomic shotgun sequencing of food
samples, could not only reduce the turnaround time of samples in an outbreak investigation …

Metagenomic sequencing analysis has been implemented as an alternative approach for
pathogen diagnosis in recent years, which is independent on cultivation and able to identify …

Background: Food contamination by pathogens poses a global health threat, affecting an
estimated 600 million people annually. During a foodborne outbreak investigation …

Outbreak investigations are essential to control and prevent the dissemination of pathogens.
This study developed and validated a complete analysis protocol for faster and more …