Application of single-cell RNA sequencing in optimizing a combinatorial therapeutic strategy in metastatic renal cell carcinoma
Background Intratumoral heterogeneity hampers the success of marker-based anticancer
treatment because the targeted therapy may eliminate a specific subpopulation of tumor …
treatment because the targeted therapy may eliminate a specific subpopulation of tumor …
Beyondcell: targeting cancer therapeutic heterogeneity in single-cell RNA-seq data
C Fustero-Torre, MJ Jiménez-Santos, S García-Martín… - Genome Medicine, 2021 - Springer
We present Beyondcell, a computational methodology for identifying tumour cell
subpopulations with distinct drug responses in single-cell RNA-seq data and proposing …
subpopulations with distinct drug responses in single-cell RNA-seq data and proposing …
Single-cell mRNA sequencing identifies subclonal heterogeneity in anti-cancer drug responses of lung adenocarcinoma cells
Background Intra-tumoral genetic and functional heterogeneity correlates with cancer
clinical prognoses. However, the mechanisms by which intra-tumoral heterogeneity impacts …
clinical prognoses. However, the mechanisms by which intra-tumoral heterogeneity impacts …
Single-cell RNA sequencing reveals the tumor microenvironment and facilitates strategic choices to circumvent treatment failure in a chemorefractory bladder cancer …
HW Lee, W Chung, HO Lee, DE Jeong, A Jo, JE Lim… - Genome medicine, 2020 - Springer
Background Tumor cell-intrinsic mechanisms and complex interactions with the tumor
microenvironment contribute to therapeutic failure via tumor evolution. It may be possible to …
microenvironment contribute to therapeutic failure via tumor evolution. It may be possible to …
Deconvolution of cell type-specific drug responses in human tumor tissue with single-cell RNA-seq
Background Preclinical studies require models that recapitulate the cellular diversity of
human tumors and provide insight into the drug sensitivities of specific cellular populations …
human tumors and provide insight into the drug sensitivities of specific cellular populations …
Deciphering the biology of circulating tumor cells through single-cell RNA sequencing: implications for precision medicine in cancer
S Orrapin, P Thongkumkoon, S Udomruk… - International Journal of …, 2023 - mdpi.com
Circulating tumor cells (CTCs) hold unique biological characteristics that directly involve
them in hematogenous dissemination. Studying CTCs systematically is technically …
them in hematogenous dissemination. Studying CTCs systematically is technically …
Personalized oncology through integrative high-throughput sequencing: a pilot study
Individual cancers harbor a set of genetic aberrations that can be informative for identifying
rational therapies currently available or in clinical trials. We implemented a pilot study to …
rational therapies currently available or in clinical trials. We implemented a pilot study to …
Overcome tumor heterogeneity-imposed therapeutic barriers through convergent genomic biomarker discovery: A braided cancer river model of kidney cancer
Tumor heterogeneity, encompassing genetic, epigenetic, and microenvironmental variables,
is extremely complex and presents challenges to cancer diagnosis and therapy. Genomic …
is extremely complex and presents challenges to cancer diagnosis and therapy. Genomic …
Optimizing cancer genome sequencing and analysis
Tumors are typically sequenced to depths of 75x–100x (exome) or 30x–50x (whole
genome). We demonstrate that current sequencing paradigms are inadequate for tumors …
genome). We demonstrate that current sequencing paradigms are inadequate for tumors …
[HTML][HTML] Advances in single-cell RNA sequencing and its applications in cancer research
Unlike population-level approaches, single-cell RNA sequencing enables transcriptomic
analysis of an individual cell. Through the combination of high-throughput sequencing and …
analysis of an individual cell. Through the combination of high-throughput sequencing and …