… gene regulation at the single-cell level. … or how gene activity or expression is modulated
in response to stimuli, we can obtain a unique and dynamic perspective of gene regulation. …

… approaches to new areas of bacterial physiology. … cell‐free expression systems derived
from eukaryotic organisms to study and engineer increasingly complex layers of gene regulation …

… value in a wide range of physiological settings. These … metabolism in addition to
transcriptional control to achieve … As enumerated in the above sections, predictive modelling has …

… important in understanding some aspect of cell physiology. The model may be relatively simple,
… To connect this abstract mathematical notion to cell physiology we must recognize that a …

… , a phenomenon in which physiological variables are … In addition, the cell-free reaction
platform makes the design … to the limitations of quantitative predictive models for the latter …

… universal gene regulatory motif that produces robust perfect adaptation for mammalian cells,
… The importance of homeostasis is clearly exemplified in physiology and medicine, where a …

… key roles in transcription regulation that are not yet fully understood. The emerging model is
that … Furthermore, in situ studies are necessary to recapitulate physiological chromatin states …

… regions of genes under its transcriptional control. We utilize biophysical methods to dissect
the binding process, statistical mechanical modeling to integrate and rationalize the results, …

… Gene expression is central to all living systems and subject to a complex regulation all the
way from RNA synthesis to … Transcriptional initiation is the most important point of control in the …

… these same kinds of predictive models can describe induction of transcription. Furthermore,
… our results entail for future efforts in understanding gene regulation. We argue that we have …