Advanced microscopy techniques for revealing molecular structure of starch granules

I Chakraborty, S Pallen, Y Shetty, N Roy… - Biophysical …, 2020 - Springer
… of starch granules is revealed by second harmonic generation (… anisotropic materials such
as starch, cellulose, and live cell … The determination of granular shape and Maltese crosses of …

Polarimetric Nonlinear Microscopy of Starch Granules

D Tokarz, R Cisek, V Barzda - Enzymology of Complex Alpha …, 2021 - api.taylorfrancis.com
… A schematic of a polarimetric second harmonic generation microscope used for starch imaging.
Detection of starch granules in a living plant by optical second harmonic microscopy. J. …

Revealing the Structural Organization of Gamma-irradiated Starch Granules Using Polarization-resolved Second Harmonic Generation Microscopy

MC Chen, I Govindaraju, WH Wang… - Microscopy and …, 2023 - academic.oup.com
… in the form of granules in plants. Approximately 98–99% of the starch granule dry weight is …
Following the same imaging scheme, the detection of X and Y polarization components when …

Second harmonic generation microscopy of polysaccharide macrostructures

R Cisek, D Tokarz - Advanced Biophysical Techniques for Polysaccharides …, 2024 - Elsevier
microscopy can be used to directly image starch granules … the forward detected SHG signal
via the microscope collection … studies of live systems were performed on starches within …

Nondestructive circadian profiling of starch content in fresh intact Arabidopsis leaf with two-photon fluorescence and second-harmonic generation imaging

JN Liao, WL Chen, CY Lo, MH Lai, HL Tsai… - Scientific Reports, 2022 - nature.com
… are two primary places where starch is produced in plants. … as a contrast mechanism in
multiphoton microscopy for imaging a … of starch granules in living cells can be effectively detected

Label-free fried starchy matrix: Investigation by harmonic generation microscopy

A Chouët, S Chevallier, R Fleurisson, C Loisel… - Sensors, 2019 - mdpi.com
Second harmonic generation (SHG) generated by starch … that starch granules could be
detected in living plants based on … interest of SHG to detect starch granules in plant seeds. Zhou …

Microfibril angle of elementary flax fibres investigated with polarised second harmonic generation microscopy

A Melelli, F Jamme, D Legland, J Beaugrand… - Industrial Crops and …, 2020 - Elsevier
… from living plants (Lantana camara) but also rice starch grains … a flax fibre with a starch granule
of spherical shape deposited … 1), but they cannot be detected and correctly visualised due …

[PDF][PDF] Quantitative Imaging of Starch Granule Development in Leaves

L Bürgy - 2021 - research-collection.ethz.ch
… and, using serial block face scanning microscopy, I extracted … The discovery of these new
factors involved in starch granule … technique to observe starch granules within a plant cell is …

Comparison of different polarization sensitive second harmonic generation imaging techniques

M Alizadeh, M Ghotbi, P Loza-Alvarez, D Merino - Methods and protocols, 2019 - mdpi.com
… of starch granules have been used. Starch granules are made of microscopic complex …
setup and (c) the detection unit to collect the second harmonic generation (SHG) signal in …

A novel apparatus for time-lapse optical microscopy of gelatinisation and digestion of starch inside plant cells

DT Do, J Singh, I Oey, H Singh, RY Yada, JM Frostad - Food Hydrocolloids, 2020 - Elsevier
… No significant changes (p > 0.05) in these cell parameters were detectedmicroscopic
imaging technique for real-time visualisation of the dynamic behaviour of trapped starch granules