Cryopreservation of Bangladeshi ram semen using different diluents and manual freezing techniques

PK Jha, MGS Alam, AAL Mansur, N Naher, T Islam… - Cryobiology, 2019 - Elsevier
… In this study three manual freezing techniques and three diluents were compared. … Analysis
of variance (ANOVA) was undertaken out using SPSS (20 Version) statistical software to …

Cryopreservation of ram semen: Manual versus programmable freezing and different lengths of equilibration

J Vozaf, AV Makarevich, A Balazi… - Animal Science …, 2021 - Wiley Online Library
vitro assay using heterologous penetration/fertilization (P/F) … rate (Table 2, Figure 3) even
after storage in liquid nitrogen. … the manual freezing of ram sperm can be applied in the field

Multiparametric study of antioxidant effect on ram sperm cryopreservation—from field trials to research bench

MF Riesco, M Alvarez, L Anel-Lopez, M Neila-Montero… - Animals, 2021 - mdpi.com
… : in vitro sperm quality analyses and in vivo field trials based on fertility. … we performed a
multiparametric analysis of some previously … effects on sperm quality during freezing and thawing …

Alternative opportunities to collect semen and sperm cells for ex situ in vitro gene conservation in sheep

MA Mujitaba, I Egerszegi, G Kútvölgyi, S Nagy, N Vass… - Agriculture, 2022 - mdpi.com
… on pre-freeze and post-thaw ram sperm quality parameters and … -target tissues in the
intrapelvic area) and non-segmented. … to test and adapt the sperm collection and storage methods

A Comparison of Freezing Methods and Diluents Types on Post-Thaw Sperm Quality of Ram Sperm

PK Jha, MGS Alam, FY Bari - Research in Agriculture Livestock and …, 2020 - banglajol.info
… of two types of freezing method and three types of diluents on post thaw sperm quality in
Bangladeshi ram semen. Many … Field and in vitro assay of three methods for freezing ram

Practical method for freezing buck semen

JM Morrell, P Malaluang, T Ntallaris, A Johannisson - Animals, 2022 - mdpi.com
… may reflect the intended use of the semen: artificial insemination (AI), in vitro fertilization (IVF), …
in 8 fields, containing at least 1000 spermatozoa, using the settings for ram spermatozoa …

[PDF][PDF] Ram semen cryopreservation using egg yolk or egg yolk-free extenders: Preliminary results

M Ptáček, M Stádníková, F Savvulidi… - Sci. Agric. Bohem, 2019 - researchgate.net
… -thawed ram spermatozoa, collected at 3 different test days (… (in terms of in vitro and in vivo
sperm function assays), some … the methodology of ram sperm freezing under field conditions …

[HTML][HTML] Two-step accelerating freezing protocol yields a better motility, membranes and DNA integrities of thawed ram sperm than three-steps freezing protocols

DA Galarza, A López-Sebastián, H Woelders… - Cryobiology, 2019 - Elsevier
… DNA fragmentation of frozen-thawed ram sperm was assessed by TUNEL assay. For this …
Each microscopic field was evaluated first under UV-light to determine the total number of …

Effect of different concentrations of resveratrol on the quality and in vitro fertilizing ability of ram semen stored at 5 C for up to 168 h

MG Al-Mutary, MQ Al-Ghadi, AA Ammari, AR Al-Himadi… - Theriogenology, 2020 - Elsevier
… The length, width and area of sperm head were lower (P ≤ … examined using repeated
measures ANOVA test. Tukey post-… to the freezing extenders improves the post-thaw sperm motility…

Freezing–thawing procedures remodel the proteome of ram sperm before and after in vitro capacitation

P Peris-Frau, A Martín-Maestro… - International journal of …, 2019 - mdpi.com
analysis was conducted between fresh and cryopreserved … regions of sperm to meet their
specific functions (Table 3). … sperm motility were evaluated subjectively using bright field