… PCR to obtain sufficient cDNA for short-length sequencing. All … , maximum subread length
= 15,000, minimum number of passes … of second-generation sequencing, the full-length reads …

… The cDNA was subsequently PCR-amplified with a cocktail of four … not correspond to the
full-length coding regions of mouse V L … This contains ≈ 15,000 sequences for rearranged, …

… called Next-Generation Sequencing (NGS) methods more than … does not provide fulllength
cDNA sequences, and transcripts … genes between 15,000 and 18,000118. Gene counts in the …

… -read/second-generation sequencing) can only attain short … on ice, the precipitate was
pelleted at 15,000 g, 4 C, 30 min. … with the mouse assembly to identify the full-length long reads, …

… With the arrival of the era of next-generation sequencing (… TAIL-seq [14], and for mouse NIH
3T3 cells the median poly(A) … poly(A) sequence information from the first cDNA strand of Poly(…

… genomes that are typically 15,000 to 19,000 nucleotides (nt) … first nucleotide of the IG region
to be important in generating … This produces a full-length, faithful, positive-sense copy of the …

… sequencing include WGS, targeted sequencing, full-length … to be converted to cDNA before
sequencing, thereby losing … in primary mouse lymphoma and human leukemia samples.[68] …

… that long-read sequencing of full-length cDNAs is essential for … splicing factors influence
isoform generation (Additional file 12: … or mouse protein databases by BLAST-P, but non-cancer-…

… development of next-generation murine models for preclinical … To this end, we screened a
cDNA library generated from the … percentages of diverse full-length mouse ORFs were pooled …

… The cDNA was subsequently PCR-amplified with a cocktail … not correspond to the full-length
coding regions of mouse V L … This contains ≈15,000 sequences for rearranged, productive…