… amidotransferases. Primary emphasis will be placed on the enzymes from Bacillus subtilis
and Escherichia coli … is well supported by studies of the purified proteins.24 25 Figure 1 shows …

… The ASE sequence of Escherichia coli was chosen as the outgroup. … coli BL21 (DE3). The
proteins were purified by nickel column affinity chromatography and desalinated in a PD10 …

… However, only the enzymes from Escherichia coli (E. coli) and Bacillus subtilis (B. subtilis)
have been … by Ni-affinity column chromatography and was further subjected to gel filtration …

… However, only the enzymes from Escherichia coli ( E. … Recombinant His-tagged protein
was purified by Ni-affinity column chromatography and was further subjected to gel filtration …

… To validate Gsk as a ppGpp target, we purified recombinant Gsk and performed isothermal
titration calorimetry (ITC). Both GDP and ppGpp bound Gsk at 1:1 stoichiometry (Figure 1B; …

… Although the purified Escherichia coli γ-glutamyltranspeptidase has much higher … coli
GGT can utilize glutamine, a relatively inexpensive amino acid, as a γ-glutamyl donor …

… by in vitro studies purifying and assaying individual enzymes … , while the glutamine
amidotransferase domain produces … class I PurE CAIR mutase of Escherichia coli. Referred to in …

… The phosphoribosyl pyrophosphate synthetase and phosphoribosyl pyrophosphate
amidotransferase … For all glutamine PRPP amidotransferase assays, 1 unit of activity is defined as …

… -5-phosphate to phosphoribosyl pyrophosphate. The reaction … coli BL21(DE3), purified
used standard immobilized metal … The movement of the glutamine residue is likely an induced …

… are reincorporated into phosphoribosyl pyrophosphate (PRPP) … Strep is an eight amino acid
tag used for the purification of … closest structural homolog from Escherichia coli, PurD (Figure …