Visualizing extracellular vesicles and their function in 3D tumor microenvironment models

EE Ural, V Toomajian, E Hoque Apu, M Veletic… - International Journal of …, 2021 - mdpi.com
Three-dimensional (3D) cell cultures can be used to study cell-to-cell and cell-to-matrix …
3D cultures may best model the role of EVs in formation of the tumor microenvironment (TME) …

[HTML][HTML] Capturing the third dimension in drug discovery: Spatially-resolved tools for interrogation of complex 3D cell models

D Simão, CM Gomes, PM Alves, C Brito - Biotechnology Advances, 2022 - Elsevier
… Advanced three-dimensional (3D) cell models have proven to be capable of depicting
architectural and microenvironmental … Moreover, fluorescent probes contributed to the functional …

The Open Challenge of in vitro Modeling Complex and Multi-Microbial Communities in Three-Dimensional Niches

M Oriano, L Zorzetto, G Guagliano… - … in bioengineering and …, 2020 - frontiersin.org
… environments embedding themselves in three-dimensional matrices, secluding from the …
the need to produce models to mimic the three-dimensional niches in which bacteria, and …

Mechanical studies of the third dimension in cancer: From 2D to 3D model

F Paradiso, S Serpelloni, LW Francis… - International Journal of …, 2021 - mdpi.com
… cell-microenvironment interactions, and plays a key role in … be measured with different
methods probing stiffness at the nano… that ovarian cells in three-dimensional (3D) culture are …

Biosensors for studies on adhesion-mediated cellular responses to their microenvironment

NA Saffioti, EA Cavalcanti-Adam… - … in Bioengineering and …, 2020 - frontiersin.org
function of the cellular microenvironment, but also to obtain quantitative information on how
cells sense and probe … conditions of cells in multicellular organizations. The incorporation of …

Micropatterning the organization of multicellular structures in 3D biological hydrogels; insights into collective cellular mechanical interactions

B Ergaz, S Goren, A Lesman - Biofabrication, 2023 - iopscience.iop.org
… hydrogel results in poor control over the cell microenvironment, due to the variability in cell
… the native microenvironment and for studies of individual cell responses to microenvironment

Transport of nanoprobes in multicellular spheroids

M Pilz, K Kwapiszewska, T Kalwarczyk, G Bubak… - Nanoscale, 2020 - pubs.rsc.org
… of the probe. Exponent a is a constant of the order of 1. … inert tracers in the three-dimensional
models of cancer as well as … by a probe undergoing motion in the ECM is a function of the …

Functions and clinical significance of mechanical tumor microenvironment: cancer cell sensing, mechanobiology and metastasis

H Zhou, M Wang, Y Zhang, Q Su, Z Xie… - Cancer …, 2022 - Wiley Online Library
… and micropillars are fundamental tools for probing the mechanical forces between cell-…
, multicellular geometry-based tumor cell detection (MGTD) and tumor microenvironment

[HTML][HTML] Emerging approaches to study cell–cell interactions in tumor microenvironment

N Nishida-Aoki, TS Gujral - Oncotarget, 2019 - ncbi.nlm.nih.gov
… the development and function of multicellular organisms. … tomography to mouse to achieve
three-dimensional, wide-field, … expression using fluorescent probes recognizing RNAs linking …

Spatio-temporal modeling and live-cell imaging of proteolysis in the 4D microenvironment of breast cancer

K Ji, M Sameni, K Osuala, K Moin, RR Mattingly… - Cancer and Metastasis …, 2019 - Springer
probes for cysteine cathepsins, as we did the live-cell proteolysis assay, to detect effects of
acidosis in the tumor microenvironment … that integrates “three-dimensional cell-culture systems…