The influence of low-and high-negative-pressure liposuction and different harvesting sites on the viability and yield of adipocytes and other nucleated cells

M Molitor, M Trávníčková, O Měšťák… - Aesthetic Plastic …, 2021 - Springer
… , b processed analysis of blood admixture, c analysis of cell … The cell density, ie the absolute
number of viable cells for … mask the influence of negative pressure on the lipoaspirate. Other …

The impact of harvesting systems and donor characteristics on viability of nucleated cells in adipose tissue: a first step towards a manufacturing process

NA Papadopulos, S Wigand, N Kuntz… - Journal of …, 2019 - journals.lww.com
source for obtaining MSC = adipose stromal cells [ASCs]. … Most of the donors provided more
than 1 lipoaspirate gained … the concentration of viable cells gained from several donor sites, …

Lipoaspirate storage time and temperature: effects on stromal vascular fraction quality and cell composition

JD Svalgaard, S Juul, PV Vester-Glovinski… - Cells Tissues …, 2020 - karger.com
… of the lipoaspirate to the processing site before SVF isolation and subsequent cell culture …
) at a final concentration of 2.5 mg/mL was added to the lipoaspirate and incubated for 45 min …

[HTML][HTML] Lipoaspirate stored at a constant low temperature by electric control suppresses intracellular metabolism and maintains high cell viability

R Inaki, Y Sato, D Nakamura, Y Aikawa, T Takato… - Regenerative …, 2023 - Elsevier
… as a promising source for cell-based therapies due to their pharmacological effects which …
BES-So was used at a concentration for 1 h in the dark (excitation, 505 nm; emission, 544 …

Correlation between tissue-harvesting method and donor-site with the yield of spheroids from adipose-derived stem cells

AB Di Stefano, E Cammarata, M Trapani… - Journal of Plastic …, 2022 - jprasurg.com
… investigated the effects of adipose tissue donor-site, harvesting … Briefly, the lipoaspirate
samples were subjected to an … showed a statistical correlation with spheroid density (p = 0.0299 …

A Novel Method to Optimize Autologous Adipose Tissue Recovery with Extracellular Matrix Preservation

I Roato, F Mussano, S Reano, F Boriani, A Margara… - Processes, 2020 - mdpi.com
… detectable Hb concentration in Lipocell-treated samples compared to untreated lipoaspirate
(… Subcutaneous adipose tissue is an abundant source of ATD-MSCs, thus it is a promising …

The cell yields and biological characteristics of stromal/stem cells from lipoaspirate with different digestion loading ratio

Z Li, D Mu, C Liu, M Xin, S Fu, S Li, J Qi, Q Wang… - Cytotechnology, 2020 - Springer
… and not influenced by contaminant red blood cells. … study although similar enzyme
concentration and oscillation time … counting methods, donor site and storage duration was …

Fat Processing Methods

A Condé-Green, AL Cansanção - Plastic and Aesthetic Regenerative …, 2022 - Springer
… as an abundant source of mesenchymal stem cells (MSCs), … yields the greatest density of
viable cells (high density fat) … choice of the donor site followed by harvesting, processing, and …

The impact of human lipoaspirate and adipose tissue-derived stem cells contact culture on breast cancer cells: Implications in breast reconstruction

A Ejaz, KS Yang, KP Venkatesh, S Chinnapaka… - International Journal of …, 2020 - mdpi.com
lipoaspirates donors to comprehensively compare the effects of different cell processing
techniques, from the same source, … SVF cells were inoculated into cell culture flasks at a density

The influence of high and low negative pressure liposuction and various harvesting techniques on the viability and function of harvested cells—a systematic review of …

M Molitor, M Trávníčková, O Měšťák… - Aesthetic Plastic …, 2021 - Springer
… assessed the cell viability and function after harvesting or processing, other … influence of
negative pressure on nucleated cells (predominantly adipocytes and ADSs) within lipoaspirate