… three llama antibody fragments (nanobodies) that bind p24, a structural HIV protein. One …
Unravelling the molecular basis of high affinity nanobodies against HIV p24: in vitro functional, …

… an anti-p24 nanobody has been employed as a detector of p24 Gag … in vitro of residues
predicted to provide higher affinity … in order to reconstruct functional antibody molecules [16,43]. …

… chemical stability, high solubility, commendable in vivo tissue … -p24 monovalent and bivalent
(41) nanobodies against HIV; … , UK, have made nanobodies (exhibiting high affinity to the S …

… was poorly released from cells, all three nanobody-Fc proteins were functional in immunoblotting
and … In vitro use of nanobody-human IgG Fc fusion proteins for immunoblotting and …

… , the nanobody regained its affinity in vitro. Using other nanobodies against Her2 and EGFR…
Usually, the intrinsic high affinity of an average monovalent nanobody (K d 10 −8 –10 −10 M) …

… present, higher affinity SARS-CoV-2 neutralizing Nbs are mainly obtained through the “in vivo”
… , and it can still maintain function after atomization, freeze-drying and heat treatment (59). …

… been carried out for an invitro identification of human anti-… protein p24 [43, 44] of human
immunodeficiency viruses 1 (HIV-1) … knowledge-based scoring function and site constraint. Then, …

… is required to achieve a high affinity interaction between Nb and … choose between an
affinity/specificity-based or a functional/… ; to a radioisotope for eg molecular in vivo imaging or to an …

… After the selection of the phages with high affinity by the … Two anti-p24 nanobodies (the p24
protein is an important … agents, and (iii) the unraveling of the structure of the spike protein (S), …

… were capable of binding p53 with high affinity in the … that nanobodies can be used to
manipulate protein function in a … some controversy about the in vivo relevance of this function. An …