Comparative analysis of exosome isolation methods using culture supernatant for optimum yield, purity and downstream applications

GK Patel, MA Khan, H Zubair, SK Srivastava… - Scientific reports, 2019 - nature.com
Exosomes have received significant attention for their role in pathobiological processes and
are being explored as a tool for disease diagnosis and management. Consequently, various …

Current status and outlook of advances in exosome isolation

W Liu, Z Ma, X Kang - Analytical and Bioanalytical Chemistry, 2022 - Springer
Exosomes are extracellular vesicles with a diameter ranging from 30 to 150 nm, which are
an important medium for intercellular communication and are closely related to the …

A comparative study of serum exosome isolation using differential ultracentrifugation and three commercial reagents

I Helwa, J Cai, MD Drewry, A Zimmerman, MB Dinkins… - PloS one, 2017 - journals.plos.org
Exosomes play a role in cell-to-cell signaling and serve as possible biomarkers. Isolating
exosomes with reliable quality and substantial concentration is a major challenge. Our …

Exosome isolation from distinct biofluids using precipitation and column-based approaches

T Soares Martins, J Catita, I Martins Rosa… - PloS one, 2018 - journals.plos.org
The potential of exosomes as biomarker resources for diagnostics, prognostics and even for
therapeutics is an area of intense research. Despite the various approaches available, there …

[HTML][HTML] Exosomes isolated from two different cell lines using three different isolation techniques show variation in physical and molecular characteristics

M Dash, K Palaniyandi, S Ramalingam… - … et Biophysica Acta (BBA …, 2021 - Elsevier
Exosomes are the nanoscopic lipid bi-layered extracellular vesicles with the potential to be
utilized as targeted therapeutics. In our investigation, we compared three major exosome …

The Yin and Yang of exosome isolation methods: conventional practice, microfluidics, and commercial kits

SZ Shirejini, F Inci - Biotechnology Advances, 2022 - Elsevier
Exosomes are a subset of extracellular vesicles released from various cells, and they can be
found in different bodily fluids. Exosomes have been utilized as biomarkers to diagnose …

Comparison of an optimized ultracentrifugation method versus size-exclusion chromatography for isolation of exosomes from human serum

M An, J Wu, J Zhu, DM Lubman - Journal of proteome research, 2018 - ACS Publications
Exosomes are nanosized vesicles that are abundant in biological fluids. In recent years,
exosomes have attracted increasing attention as their cargo may provide promising …

Research development on exosome separation technology

WM Xu, A Li, JJ Chen, EJ Sun - The Journal of Membrane Biology, 2023 - Springer
Exosomes are special extracellular vesicles secreted by cells, which are of great
significance in the basic research of life science and clinical application and has become a …

An improvised one-step sucrose cushion ultracentrifugation method for exosome isolation from culture supernatants of mesenchymal stem cells

S Gupta, S Rawat, V Arora, SK Kottarath… - Stem cell research & …, 2018 - Springer
Abstract Background Exosomes are nanovesicles (30–120 nm) of endosomal origin. These
exosomes contain various functional proteins and RNAs that could be used for therapeutic …

Advances in exosomes technology

BY Chen, CWH Sung, C Chen, CM Cheng, DPC Lin… - Clinica chimica acta, 2019 - Elsevier
Exosomes, also called extracellular vesicles (EVs), are membranous structures measuring
between 40 and 100 nm. Exosomes, secreted by various cells of the human body into body …