… important for factor binding, and regulatory networks controlling gene expression. In this
review, we discuss techniques for determining DNA accessibility and nucleosome positioning (…

… proximity, functional transcription factor binding and regulatory … to perform genome-wide
profiling of accessible chromatin 35,… nucleosome positioning and accessible chromatin regions. …

… positioning in the Saccharomyces cerevisiae genome requires … DNA accessibility is a
hallmark of active regulatory regions … Recent advances in the ability to rapidly perturb and profile …

… level and genome scale is lacking. Here we describe a … factor regulators using deviations
of transcription factor motifs (… localizing to accessible chromatin are shown. Scale bar, 25 kb. …

… a variety of techniques to quantify genome-wide accessibility, … validated the localized increases
in accessibility at regulatory … This technique has mostly been used to profile the genomic …

… ] together with myriad specific studies confirmed that TFs localize preferentially at genomic
sites containing their in vitro bound motifs. However, only a small fraction (~1% in mammalian …

… nucleosome positioning co-determine protein access to DNA in … DNA would be accessible
to an average-sized DNA binding … by DNA repair factors is essential for genome stability and …

… used method to obtain a genome-wide snapshot of chromatin … , signatures of active transcription
and transcription factor (TF… to intracellular protein detection, localized transposition (for …

… transcriptionally accessible genome (ie, ~2% of 2.5 Gbp 24 ). … A key question is whether
promiscuous DCB is just localized … Before embarking on large-scale DNA sequence profiling, …

… visualize the localization of their protein of interest at annotated genomic locations … factor
of interest occupancies could reveal connections between differences in chromatin accessibility …