… Reduction of disulfides The determination of total GSH, GSH disulfides (GSSG and GSH-mixed
disulfides) and protein bound GSH requires a reduction step, prior to derivatization, to …

… In this endpoint assay, the first absorbance was taken before the mixing … inflammatory
processes and decreased antioxidant activity [48]. … Determination of thiols and disulfides via HPLC …

… -l-cysteine disulfide is not a substrate for glutathione synthetase. L-… synthetase assay,
glutathione synthetase may be assayed … The assay mix above for glutathione synthetase minus …

… in the analysis of glutathione, and prevents underestimation of its quantity. The analysis of
real … The injection volume was 2 µL for wine and 0.4 µL mixed with 1.6 µL of formic acid 5% for …

… by the formation of GSH-mixed disulfides. Furthermore, other … [48,49] pointed out that the
total glutathione content in the … can be glutathione, allowing the formation of a mixed disulfide, …

… The derivatization reaction yields a mixed disulfide of the … protein disulfides, and consequently,
thiol disulfide exchange … for glutathione determination in tissues and biological fluids of …

… attractive potential in fluorescent analysis and bioimaging, and … 6 mL of 100 mM glutathione
disulfide were mixed with 66 mL of … GSSG differs from GSH chemically [48]. The more flexible …

… Thus, we want to develop methods of chromatographic separation followed by spectrophotometric
detection of the mixed disulfide (rather than the chromogenic thiol as in this work). …

… We demonstrate that oxidation of protein disulfides induces … Solvents for HPLC and MS
analysis and secondary antibodies … –80 μM H 2 O 2 have been reported in blood or plasma [48]). …

… by one-way analysis of variance (ANOVA) with Dunnett's post-hoc testing. Data (expressed …
give glutathionylated species [47,48]. In comparison, the role of disulfides in the formation of …