H Cao, Y Zhang, Y Cai, L Tang, F Gao, D Xu… - Frontiers in Molecular …, 2022 - frontiersin.org
… the genome landscapes of … strategy such that both polyA+ and polyA−transcripts are included. The library construction and the Illumina sequencing using a paired-end 150 bp strategy …
… Amplicon pools were subject to Illumina NovaSeq 6000 (150 PE) sequencing, and on average 20 M paired-end reads were obtained from each replicate of a sample, with high …
… aimed to reconstruct transcripts from paired-end short reads. … is the inaccurate annotation of transcriptstart sites (TSS) and … sequenced, with an average of 7 million aligned paired-end …
… -seq reveals a rich landscape of TSSs associated with Pol III transcripts that have not … sequenced on Illumina MiSeq (75-bp paired-endsequencing). The corresponding sequencing …
R Rouco, O Bompadre, A Rauseo, O Fazio… - Nature …, 2021 - nature.com
… Immediately, gel beads are dissolved and cDNA is amplified via PCR followed by library construction and sequencing. Libraries were paired-endsequenced on a HiSeq 4000. On …
… of gene expression (CAGE) sequencing, we show that … shaping the plant transcriptioninitiation landscape, we employ … reported by the pairedend analysis of transcriptionstart site (PEAT…
… in preventing cryptic transcriptioninitiation in … sequencing were performed by the Integrated Genomics Operation Core Facility at MSKCC on a HiSeq 2500 in a 50-bp/50-bp paired-end …
… of T7 promoter transcriptionstart site) … paired-endsequenced on a NovaSeq 6000 SP v1.5 flow cell using a modified run setup (see Table S2) to capture all necessary index sequence …
… that sequencing runs are carried in a paired-end format, first, … to see ATAC-seq libraries sequenced as 2×75mers or longer … profile curve around the transcriptionstart sites of protein …