Epigenetic patterns in a complete human genome

A Gershman, MEG Sauria, X Guitart, MR Vollger… - Science, 2022 - science.org
… least two ENCODE cell lines at the transcriptional start site at 57 of these … paired-end
sequencing data and at least one matching input control. These criteria yielded 96 total sequencing

[HTML][HTML] Hotspots of single-strand DNA “breakome” are enriched at transcriptional start sites of genes

H Cao, Y Zhang, Y Cai, L Tang, F Gao, D Xu… - Frontiers in Molecular …, 2022 - frontiersin.org
… the genome landscapes of … strategy such that both polyA+ and polyA−transcripts are
included. The library construction and the Illumina sequencing using a paired-end 150 bp strategy

Quantitative analysis of transcription start site selection in Saccharomyces cerevisiae reveals control by DNA sequence, RNA Polymerase II activity, and NTP levels

Y Zhu, IO Vvedenskaya, SH Sze, BE Nickels… - bioRxiv, 2021 - biorxiv.org
… Amplicon pools were subject to Illumina NovaSeq 6000 (150 PE) sequencing, and on
average 20 M paired-end reads were obtained from each replicate of a sample, with high …

[HTML][HTML] Bookend: precise transcript reconstruction with end-guided assembly

MA Schon, S Lutzmayer, F Hofmann, MD Nodine - Genome biology, 2022 - Springer
… aimed to reconstruct transcripts from paired-end short reads. … is the inaccurate annotation
of transcript start sites (TSS) and … sequenced, with an average of 7 million aligned paired-end

Comprehensive determination of transcription start sites derived from all RNA polymerases using ReCappable-seq

B Yan, G Tzertzinis, I Schildkraut, L Ettwiller - Genome research, 2022 - genome.cshlp.org
… -seq reveals a rich landscape of TSSs associated with Pol III transcripts that have not …
sequenced on Illumina MiSeq (75-bp paired-end sequencing). The corresponding sequencing

[HTML][HTML] Cell-specific alterations in Pitx1 regulatory landscape activation caused by the loss of a single enhancer

R Rouco, O Bompadre, A Rauseo, O Fazio… - Nature …, 2021 - nature.com
… Immediately, gel beads are dissolved and cDNA is amplified via PCR followed by library
construction and sequencing. Libraries were paired-end sequenced on a HiSeq 4000. On …

[HTML][HTML] Epigenetic regulation of spurious transcription initiation in Arabidopsis

NT Le, Y Harukawa, S Miura, D Boer, A Kawabe… - Nature …, 2020 - nature.com
… of gene expression (CAGE) sequencing, we show that … shaping the plant transcription initiation
landscape, we employ … reported by the paired end analysis of transcription start site (PEAT…

SETD2 loss perturbs the kidney cancer epigenetic landscape to promote metastasis and engenders actionable dependencies on histone chaperone complexes

Y Xie, M Sahin, S Sinha, Y Wang, AM Nargund, Y Lyu… - Nature cancer, 2022 - nature.com
… in preventing cryptic transcription initiation in … sequencing were performed by the Integrated
Genomics Operation Core Facility at MSKCC on a HiSeq 2500 in a 50-bp/50-bp paired-end

High-throughput single-cell epigenomic profiling by targeted insertion of promoters (TIP-seq)

DA Bartlett, V Dileep, T Handa, Y Ohkawa… - Journal of Cell …, 2021 - rupress.org
… of T7 promoter transcription start site) … paired-end sequenced on a NovaSeq 6000 SP v1.5
flow cell using a modified run setup (see Table S2) to capture all necessary index sequence

Interrogating the accessible chromatin landscape of eukaryote genomes using ATAC-seq

GK Marinov, Z Shipony - Deep Sequencing Data Analysis, 2021 - Springer
… that sequencing runs are carried in a paired-end format, first, … to see ATAC-seq libraries
sequenced as 2×75mers or longer … profile curve around the transcription start sites of protein …