Global landscape of replicative DNA polymerase usage in the human genome

E Koyanagi, Y Kakimoto, T Minamisawa… - Nature …, 2022 - nature.com
… termination close to transcription start sites and show that transcription can perturb the …
libraries for Illumina sequencing. Approximately 200 million paired-end reads were obtained per …

Genome-wide mapping of i-motifs reveals their association with transcription regulation in live human cells

I Zanin, E Ruggiero, G Nicoletto, S Lago… - Nucleic Acids …, 2023 - academic.oup.com
… at gene promoters, close to the transcription start site (TSS), but … Samples were sequenced
paired-end on an Illumina … Here, we investigated and compared the iM- and G4-landscape

Spatially resolved epigenome sequencing via Tn5 transposition and deterministic DNA barcoding in tissue

N Farzad, A Enninful, S Bao, D Zhang, Y Deng… - Nature Protocols, 2024 - nature.com
… region-specific epigenetic landscapes in mouse embryos … are usually found proximal to the
transcription start site (TSS) … are sequenced using paired-end sequencing corresponding to …

Combining TSS-MPRA and sensitive TSS profile dissimilarity scoring to study the sequence determinants of transcription initiation

C Guzman, S Duttke, Y Zhu… - Nucleic Acids …, 2023 - academic.oup.com
… the transcription initiation landscape of endogenous sequences, we developed a windowed
… by paired-end sequencing and aligning the cloned sequences to the original sequences

Single-cell chromatin accessibility maps reveal regulatory programs driving early mouse organogenesis

B Pijuan-Sala, NK Wilson, J Xia, X Hou, RL Hannah… - Nature cell …, 2020 - nature.com
… To explore the resulting chromatin landscape, we defined open … resulting peak list with
known transcription start sites (TSS) to … were sequenced with NextSeq500 (three 50 paired-end

From reads to insight: a hitchhiker's guide to ATAC-seq data analysis

F Yan, DR Powell, DJ Curtis, NC Wong - Genome biology, 2020 - Springer
… to decipher the epigenetic landscape. These include Assay … Paired-end sequencing is then
performed to facilitate higher … to be enriched around the transcription start site (TSS) of genes, …

RNA structure landscape of S. cerevisiae introns

R Rangan, R Huang, O Hunter, P Pham, M Ares Jr… - bioRxiv, 2022 - biorxiv.org
… find the most prominent transcription start site upstream of the … sequences and ensuring
consistency between paired-end … We then merged paired-end reads using bbmerge 101 with …

Identification of plant transcription factor DNA-binding sites using seq-DAP-seq

S Hutin, R Blanc-Mathieu, P Rieu, F Parcy, X Lai… - Plant gene regulatory …, 2023 - Springer
… are PCR-amplified, barcoded if necessary, and sequenced using paired-end sequencing
TF, with sequencing data from an ampDAP-seq experiment with paired end reads with two …

Chromatin accessibility profiling methods

L Minnoye, GK Marinov, T Krausgruber, L Pan… - Nature Reviews …, 2021 - nature.com
… a chromatin landscape is shown in which transcriptionsequencing (ChIP–seq) and related
methods, as these are powerful techniques to gain further insights into chromatin landscapes

Precise transcript reconstruction with end-guided assembly

MA Schon, S Lutzmayer, F Hofmann, MD Nodine - bioRxiv, 2022 - biorxiv.org
transcript start and end sites is essential for precise transcript … aimed to reconstruct transcripts
from paired-end short reads. … sequenced, with an average of 7 million aligned paired-end