Selection of broad-spectrum aptamer and its application in fabrication of aptasensor for detection of aminoglycoside antibiotics residues in milk

F Yue, H Li, Q Kong, J Liu, G Wang, F Li, Q Yang… - Sensors and Actuators B …, 2022 - Elsevier
… The ssDNA that bound to targets was purified and then used as PCR template for … In
order to validate the reliability and applicability of this aptasensor, the liquid chromatography-…

An Aptamer Affinity Column for Extraction of Four Aminoglycoside Antibiotics from Milk

L Zhao, X Jiang, X Xu, N Wang, X Wang, R Yang, X Liu… - Separations, 2022 - mdpi.com
… column may lead to poor purification effect, poor detection sensitivity, and poor recovery …
hydrophilic interaction chromatography, more and more researchers tend to use hydrophilic …

Advances in the application of aptamer biosensors to the detection of aminoglycoside antibiotics

Y Luan, N Wang, C Li, X Guo, A Lu - Antibiotics, 2020 - mdpi.com
… methods include high performance liquid chromatography (LC) [23,… , the use of LC-MS for
AG detection requires the use of … Generally, solid-phase extraction is used to extract and purify

Split aptamer acquisition mechanisms and current application in antibiotics detection: A short review

H Ye, Z Yang, IM Khan, S Niazi, Y Guo… - Critical Reviews in …, 2023 - Taylor & Francis
… lack of review on antibiotic-related split aptamer and their … application of split aptamer in
antibiotic analysis were discussed and summarized, and the future development of split aptamer

Electrochemical aptasensors for antibiotics detection: Recent achievements and applications for monitoring food safety

G Evtugyn, A Porfireva, G Tsekenis, V Oravczova… - Sensors, 2022 - mdpi.com
… Conventional methods of antibiotics detection such are microbiological assays chromatographic
… acid aptamers for antibiotic detection. A brief explanation of conventional methods of …

Dual-mode sensor based on GO-SELEX screening of aptamer for detecting cefapirin antibiotic residues in milk

Z Sun, W Zhang, Y Mou, Y Tian, H Dong, X Sun… - Sensors and Actuators B …, 2024 - Elsevier
… The ssDNA-enriched sequences obtained in the 13th round of screening were PCR amplified,
purified, and sent to Shanghai Sangon Biotech Co., Ltd. for cloning and sequencing. The …

[HTML][HTML] Recent advances in aptamer-based miniaturized extraction approaches in food analysis

M Vergara-Barberan, MJ Lerma-Garcia, A Moga… - TrAC Trends in …, 2021 - Elsevier
… in the isolation and determination of several compounds … (POPs), antibiotics, toxins and
bacteria in different food matrices… liquid-chromatography (HPLC) using fluorescence detection (…

Screening of broad-spectrum aptamer and development of electrochemical aptasensor for simultaneous detection of penicillin antibiotics in milk

M Hu, F Yue, J Dong, C Tao, M Bai, M Liu, S Zhai… - Talanta, 2024 - Elsevier
… At present, many detection methods of antibiotics have been … , high performance liquid
chromatography (HPLC), microchip … Screening was stopped at 13th round, and the purified PCR …

Fabrication of magnetic molecularly imprinted polymers based on aptamers and β-cyclodextrin for synergistic recognition and separation of tetracycline

Y Ma, X Liao, Y Zhao, L Qiu, Y Yao, S Wang… - Analytica Chimica …, 2022 - Elsevier
… Tetracycline is extensively used as an antibiotic in animal … high-performance liquid
chromatography to detect ultra-trace … effect of extraction buffers on the adsorption capacity, various

Using target-specific aptamers to enhance the peroxidase-like activity of gold nanoclusters for colorimetric detection of tetracycline antibiotics

Z Zhang, Y Tian, P Huang, FY Wu - Talanta, 2020 - Elsevier
… high performance liquid chromatography (HPLC), gas chromatography-mass spectrometry
(… reagent grade and used without further purification. TC-binding aptamers used in this study (…