… of PERV inactivation for XTx, we tested the susceptibility of a PERV RT-inactivated porcine
… PERV and investigated the influence of CRISPR/Cas9-mediated indels on virus particle …

… PERVs raised the question of whether conventional pigs can still be used for xenotransplantation,
or whether only CRISPR/Cas9 inactivated pigs must be used as source animals for …

… Pig to human xenotransplantation is considered to be a possible … Porcine endogenous
retrovirus (PERV) is the most significant pathogen in xenotransplantation. We screened for pigs …

… Furthermore, we applied CRISPR/Cas9 technology to inactivate and eliminate the
potential risk of cross-species transmission and obtained PERV-destructed PRF clonal cells. …

… endogenous retroviruses (PERVs) could infect human cells. In the past, we determined
the … the porcine kidney epithelial cell line PK15 and in primary fibroblasts. Using CRISPR-Cas9, …

… Our major goal in this study was to explore the possibility of producing pRSAD2-KI pigs via
SCNT. First, the CRISPR/Cas9 vectors and the pRSAD2 targeting vectors were introduced …

… of swine viral genes and host factors related to their infections, improving the antiviral ability
of pigs, inactivating porcine endogenous retrovirus … study utilizing CRISPR-Cas9 technology …

… the innovations in swine research mediated by the advent of the CRISPR/Cas9 gene editing.
… encountered with CRISPR/Cas9 gene editing and its future applications in swine research. …

… CRISPR/Cas9 has been used to inactivate PERVs. However, Cas9 also triggers severe DNA
damage at multiple integrated PERV sites in the porcine … -knockout pigs using base editors. …

… However, immune rejection and the potential risk of porcine endogenous retroviruses (PERVs)
are two major hurdles that restrict the development of xenotransplantation protocols. …