Improved sample preparation method for fast LC-MS/MS analysis of vitamin D metabolites in serum

R Rola, K Kowalski, T Bieńkowski… - … and Biomedical Analysis, 2020 - Elsevier
… , PP is only an introduction to the further stages of sample preparation, mainly using LLE or
… a fast serum sample preparation method based on PP alone to efficiently extract vitamin D

Sample preparation techniques for extraction of vitamin D metabolites from non-conventional biological sample matrices prior to LC–MS/MS analysis

A Alexandridou, DA Volmer - Analytical and Bioanalytical Chemistry, 2022 - Springer
… and efficient sample preparation protocols for sample matrices … sample preparation methods
for measurement of vitamin D … milk samples to determine vitamin D sulphated analogues (…

Analytical methods for 25-hydroxyvitamin D: advantages and limitations of the existing assays

A Tripathi, MS Ansari, P Dandekar, R Jain - The Journal of nutritional …, 2022 - Elsevier
… However, the absence of sample preparation in automated methods can result in the … This
review presents a summary of the latest advances in the analysis of vitamin D and its analogs

Vitamin D metabolites: analytical challenges and clinical relevance

N Alonso, S Zelzer, G Eibinger, M Herrmann - Calcified tissue international, 2023 - Springer
… established and new vitamin D related biomarkers including their pathophysiological role,
preanalytical and analytical aspects, … sample preparation that cleans the sample and extracts …

Recent advances in the analysis of vitamin D and its metabolites in food matrices

B Socas-Rodríguez, M Sandahl, C Holm, C Turner - separations, 2020 - mdpi.com
Vitamin D and its analogues are fat-soluble vitamins that carry out … vitamin D analogues
are present in such samples, make necessary the application of effective sample preparation

Analysis of vitamin D metabolic markers by mass spectrometry: Recent progress regarding the “gold standard” method and integration into clinical practice

A Alexandridou, P Schorr, CS Stokes… - Mass Spectrometry …, 2023 - Wiley Online Library
sample matrices analyzed for an increasing number of different vitamin D metabolites, novel
sample preparation techniques, … concentrations of vitamin D and its analogues; avoid issues …

Vitamin D: Current challenges between the laboratory and clinical practice

L Máčová, M Bičíková - Nutrients, 2021 - mdpi.com
vitamin D in biological samples requires pre-analytical treatment to reduce the adverse effects
of the matrix. Different methods and conditions are used for sample preparationanalogs

Vitamin D: Sources, physiological role, biokinetics, deficiency, therapeutic use, toxicity, and overview of analytical methods for detection of vitamin D and its …

J Janoušek, V Pilařová, K Macáková… - Critical reviews in …, 2022 - Taylor & Francis
… Currently, the therapeutic administration of vitamin D 3 and its analogues is considered …
LC-MS quantification to compensate for losses during the sample preparation step, prevent …

The measurement of vitamin D metabolites part II—the measurement of the various vitamin D metabolites

K Makris, C Sempos, E Cavalier - Hormones, 2020 - Springer
… measurements over time, location, and laboratory procedure”. The current NIST RMPs
measure 25(OH)D3 and 25(OH)D2 in one sample preparation and two separate LC analyses. …

Diagnostic aspects of vitamin D: clinical utility of vitamin D metabolite profiling

G Jones, M Kaufmann - Journal of Bone and Mineral Research …, 2021 - academic.oup.com
… , (2) vitamin D analogs, (3) and rapid actions of vitamin D. (4) … /MS method that uses DMEQ
TAD to derivatize the vitamin D … different vitamin D forms in a 25 to 100 μL serum sample in a …