Introducing a single‐cell‐derived human mesenchymal stem cell line expressing hTERT after lentiviral gene transfer

W Böcker, Z Yin, I Drosse, F Haasters… - Journal of cellular …, 2008 - Wiley Online Library
Human mesenchymal stem cells (hMSCs) can be readily isolated from bone marrow and
differentiate into multiple tissues, making them a promising target for future cell and gene …

Maintenance of differentiation potential of human bone marrow mesenchymal stem cells immortalized by human telomerase reverse transcriptase gene despite of …

BM Abdallah, M Haack-Sørensen, JS Burns… - Biochemical and …, 2005 - Elsevier
Human bone marrow mesenchymal stem cells (hMSC) represent a population of stem cells
that are capable of differentiation into multiple lineages. However, these cells exhibit …

Human Bone Marrow–Derived Mesenchymal Stem Cells Do Not Undergo Transformation after Long-term In vitro Culture and Do Not Exhibit Telomere Maintenance …

ME Bernardo, N Zaffaroni, F Novara, AM Cometa… - Cancer research, 2007 - AACR
Significant improvement in the understanding of mesenchymal stem cell (MSC) biology has
opened the way to their clinical use. However, concerns regarding the possibility that MSCs …

Expression of telomerase extends longevity and enhances differentiation in human adipose tissue-derived stromal cells

ES Jun, TH Lee, HH Cho, SY Suh… - Cellular Physiology and …, 2004 - karger.com
Human bone marrow stromal cells (hBMSCs) are defined as pluripotent progenitor cells with
the ability to differentiate into osteoblasts, chondrochytes, adipocytes, muscle cells, and …

Telomerase expression extends the proliferative life-span and maintains the osteogenic potential of human bone marrow stromal cells

JL Simonsen, C Rosada, N Serakinci, J Justesen… - Nature …, 2002 - nature.com
Human bone marrow stromal cells (hMSCs) were stably transduced by a retroviral vector
containing the gene for the catalytic subunit of human telomerase (hTERT). Transduced …

Clonal heterogeneity in differentiation potential of immortalized human mesenchymal stem cells

T Okamoto, T Aoyama, T Nakayama… - Biochemical and …, 2002 - Elsevier
Mesenchymal stem cells (MSCs) are bone marrow stroma-derived cells, which can
differentiate into several types of mesenchymal tissues. Although regarded as tissue-specific …

Tissue distribution and engraftment of human mesenchymal stem cells immortalized by human telomerase reverse transcriptase gene

JF Bentzon, K Stenderup, FD Hansen… - Biochemical and …, 2005 - Elsevier
Engraftment of mesenchymal stem cells (MSC) in peripheral tissues for replenishing of local
stem cell function has been proposed as a therapeutic approach to degenerative diseases …

Ectopically hTERT expressing adult human mesenchymal stem cells are less radiosensitive than their telomerase negative counterpart

N Serakinci, R Christensen, J Graakjaer… - Experimental cell …, 2007 - Elsevier
During the past several years increasing evidence indicating that the proliferation capacity of
mammalian cells is highly radiosensitive, regardless of the species and the tissue of origin of …

Molecular basis of immortalization of human mesenchymal stem cells by combination of p53 knockdown and human telomerase reverse transcriptase overexpression

TM Liu, WM Ng, HS Tan, D Vinitha, Z Yang… - Stem cells and …, 2013 - liebertpub.com
Mesenchymal stem cells (MSCs) represent one of the most promising stem cells for a
number of degenerative conditions due to their multipotency, immunoprivileged properties …

Development and functional characterization of human bone marrow mesenchymal cells immortalized by enforced expression of telomerase

K Mihara, C Imai, E Coustan‐Smith… - British journal of …, 2003 - Wiley Online Library
To create immortal mesenchymal cell lines, we transduced primary human bone marrow
mesenchymal cells with telomerase reverse transcriptase (TERT). TERT+ mesenchymal …