Construction of human telomerase catalytic subunit gene lentiviral expression vector and preliminary study on transfection of human bone marrow stromal cells

Z Chen - Chinese Journal of Tissue Engineering Research, 2012 - cjter.com
BACKGROUND: Bone marrow stromal cells (BMSCs) lack telomerase activity. Therefore,
cell telomere length gradually shortens while proliferation in vitro, resulting in cellular …

Significant prolongation of reproductive activity and life period in hMSCs by hTERT transfection

S Dongwen, D Fei, C Jun, C Xiwei, H Weifeng… - 2010 - europepmc.org
To investigate the expression of exogenous human telomerase reverse transcriptase hTERT
in the human mesenchymal stem cells hMSCs and detect the effect of the gene on cellular …

Gene expression of human telomerase reverse transcriptase in human bone marrow mesenchymal stem cells

K LI, R Liu, X Han, L MA, Y Xing - Chinese Journal of Tissue …, 2007 - pesquisa.bvsalud.org
BACKGROUND: Human mesenchymal stem cells (hMSCs) become aging and even die
after several passages. Some investigations have shown that telomere has a close …

Study of hTERT activating telomerase in human bone marrow mesenchyme stem cells

Y Teng, Y HU, H Wang - Orthopedic Journal of China, 2006 - pesquisa.bvsalud.org
[Objective] To explicate whether the telomerase activity is regulated by human telomerase
reverse transcriptase (hTERT) in human bone marrow mesenchyme stem cells …

[PDF][PDF] Ectopic hTERT gene expression in human bone marrow mesenchymal stem cell

K Li, H Zhu, X Han, Y Xing - Life Sci J, 2007 - lifesciencesite.com
Objective. To investigate how mesenchymal stem cell (MSC) telomerase activity and life
span changes after introducing the human telomerase reverse transcriptase (hTERT) gene …

Construction of Recombinant Telomerase Reverse Transcriptase Gene Lentiviral Expression Vector and Virus Packaging

Y ZHONG, R LIN, D LI, X HU… - China …, 2011 - manu60.magtech.com.cn
Objective: The aim is to construct lentiviral vector containing GFP reporter gene driven by
human telomerase reverse transcriptase (hTERT) gene and to establish high-titer lentiviral …

Construction of an eukaryotic expression vector carrying amino acids 936-1132 of human telomerase reverse transcriptase and its expression in 293T cells

J Lu, H Jiang, Y Cao, J Pang - Chinese Journal of Tissue Engineering …, 2012 - cjter.com
BACKGROUND: Human telomerase reverse transcriptase is an important component of
telomerase. The display on telomerase activity results in anti-telomere shortening or cell …

Telomerase expression during induced differentiation of bone marrow mesenchymal stem cells into neural stem cells

T Xu, M Yang, W Sheng, K Huang - Chinese Journal of Tissue …, 2012 - cjter.com
BACKGROUND: Telomerase plays an important role in cell differentiation and activation.
OBJECTIVE: To detect the expression of telomerase during induced differentiation of bone …

Ectopic hTERT gene expression in human bone marrow mesenchymal stem cells

L Ke, L Ruimin, H Xuefei, M Lan, X Ying - 2010 - europepmc.org
To investigate the effect of transfection of hTERT gene into human mesenchymal stem cells
MSCs on their telomerase activity and lifespan. METHODS Human MSCs were transfected …

Construction and identification of recombinant adenovirus vector of human telomerase reverse transcriptase

Z Peng, Z Jia, H Tang, H Huang, X Guo, Y Wei… - Chinese Journal of …, 2012 - cjter.com
BACKGROUND: Human telomerase reverse transcriptase (hTERT) closely correlates with c-
myc in laryngeal squamous cell carcinoma. OBJECTIVE: To construct recombinant …